Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
1976-9-2
pubmed:abstractText
Fluorimetric titrations were used to determine apparent dissociation constants of the all-trans isomers of retinol, retinoic acid, retinyl acetate and retinyl palmitate to human-retinol binding protein and chicken-retinol binding protein. Enhancement of the fluorescence of retinol and retinyl acetate when bound to the protein was utilized to establish the binding affinity of these compounds. With retinoic acid which is essentially a non-fluorescent compound, quenching of protein fluorescence due to energy transfer to the bound ligand from tryptophanyl residues served to determine the binding affinity. The various ligands display 1:1 molecular complexes with both types of retinol binding proteins. Retinol, retinoic acid and retinyl acetate were found to have similar binding affinities to both species of carrier proteins: For retinol K'd=1.9 X 10(-7) M with human-retinol binding protein and K'd=1.5 X 10(-7) M with chicken-retinol binding protein; for retinoic acid K'd-2.1 X 10(-7) M with human-retinol binding protein; for retinyl acetate and K'd=2.2 X 10(-7) M with chicken-retinol binding protein; for retinyl acetate K'd=2.2 X 10(-7) M with human-retinol binding protein and K'd=1.7 X 10(-7) M with chicken-retinol binding protein. Retinyl palmitate appeared to have weak association with either of the two retinol binding proteins, if at all. The above results suggest that both human and chicken retinol binding proteins behave similar with respect to the binding of the ligands. Non-polar interactions probably play a primary role in the binding and effects of functional groups and charges are of secondary importance.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
May
pubmed:issn
0014-2956
pubmed:author
pubmed:issnType
Print
pubmed:day
17
pubmed:volume
65
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
71-8
pubmed:dateRevised
2009-10-27
pubmed:meshHeading
pubmed:year
1976
pubmed:articleTitle
Binding affinities of retinol and related compounds to retinol binding proteins.
pubmed:publicationType
Journal Article