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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
1-2
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pubmed:dateCreated |
1998-2-25
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pubmed:abstractText |
Described is a method for the determination of orotic acid as its methyl ester in human urine. The method involves the use of solid-phase extraction to isolate pyrimidines from urine and derivatization with methanol and sulfuric acid, followed by isocratic high-performance liquid chromatography on a reversed-phase C18 column with UV absorbance detection. The assay is shown to be sufficiently sensitive for use in clinical investigations where elevated orotic acid excretion is suspected.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Dec
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pubmed:issn |
1387-2273
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
5
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pubmed:volume |
703
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
263-6
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pubmed:dateRevised |
2009-11-19
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pubmed:meshHeading |
pubmed-meshheading:9448085-Adult,
pubmed-meshheading:9448085-Breast Feeding,
pubmed-meshheading:9448085-Chromatography, High Pressure Liquid,
pubmed-meshheading:9448085-Creatinine,
pubmed-meshheading:9448085-Female,
pubmed-meshheading:9448085-Humans,
pubmed-meshheading:9448085-Infant, Newborn,
pubmed-meshheading:9448085-Infant Food,
pubmed-meshheading:9448085-Linear Models,
pubmed-meshheading:9448085-Methylation,
pubmed-meshheading:9448085-Ornithine Carbamoyltransferase,
pubmed-meshheading:9448085-Ornithine Carbamoyltransferase Deficiency Disease,
pubmed-meshheading:9448085-Orotic Acid,
pubmed-meshheading:9448085-Reproducibility of Results,
pubmed-meshheading:9448085-Sensitivity and Specificity,
pubmed-meshheading:9448085-Spectrophotometry, Ultraviolet
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pubmed:year |
1997
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pubmed:articleTitle |
High-performance liquid chromatographic determination of orotic acid as its methyl derivative in human urine.
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pubmed:affiliation |
Department of Clinical Medicine, Pathology and Pharmacology, University of Perugia, Italy.
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pubmed:publicationType |
Journal Article,
Comparative Study
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