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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
2
|
| pubmed:dateCreated |
1998-3-30
|
| pubmed:abstractText |
PAR-2 is a second member of a novel family of G-protein-coupled receptors characterized by a proteolytic cleavage of the amino terminus, thus exposing a tethered peptide ligand that autoactivates the receptor. The physiological and/or pathological role(s) of PAR-2 are still unknown. This study provides tissue-specific cellular localization of PAR-2 in normal human tissues by immunohistochemical techniques. A polyclonal antibody, PAR-2C, was raised against a peptide corresponding to the amino terminal sequence SLIGKVDGTSHVTGKGV of human PAR-2. Significant PAR-2 immunoreactivity was detected in smooth muscle of vascular and nonvascular origin and stromal cells from a variety of tissues. PAR-2 was also present in endothelial and epithelial cells independent of tissue type. Strong immunolabeling was observed throughout the gastrointestinal tract, indicating a possible function for PAR-2 in this system. In the CNS, PAR-2 was localized to many astrocytes and neurons, suggesting involvement of PAR-2 in neuronal function. A role for PAR-2 in the skin was further supported by its immunolocalization in the epidermis. PAR-2C antibody exemplifies an important tool to address the physiological role(s) of PAR-2.
|
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical | |
| pubmed:status |
MEDLINE
|
| pubmed:month |
Feb
|
| pubmed:issn |
0022-1554
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:volume |
46
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
157-64
|
| pubmed:dateRevised |
2004-11-17
|
| pubmed:meshHeading |
pubmed-meshheading:9446822-Amino Acid Sequence,
pubmed-meshheading:9446822-Antibody Specificity,
pubmed-meshheading:9446822-Blood Platelets,
pubmed-meshheading:9446822-Brain Chemistry,
pubmed-meshheading:9446822-Cells, Cultured,
pubmed-meshheading:9446822-Digestive System,
pubmed-meshheading:9446822-Endothelium,
pubmed-meshheading:9446822-Epidermis,
pubmed-meshheading:9446822-Epithelial Cells,
pubmed-meshheading:9446822-Humans,
pubmed-meshheading:9446822-Immunohistochemistry,
pubmed-meshheading:9446822-Molecular Sequence Data,
pubmed-meshheading:9446822-Muscle, Smooth,
pubmed-meshheading:9446822-Neurons,
pubmed-meshheading:9446822-Organ Specificity,
pubmed-meshheading:9446822-Receptor, PAR-2,
pubmed-meshheading:9446822-Receptors, Cell Surface,
pubmed-meshheading:9446822-Stromal Cells
|
| pubmed:year |
1998
|
| pubmed:articleTitle |
Characterization of protease-activated receptor-2 immunoreactivity in normal human tissues.
|
| pubmed:affiliation |
Drug Discovery Research, The R.W. Johnson Pharmaceutical Research Institute, Spring House, Pennsylvania 19477, USA.
|
| pubmed:publicationType |
Journal Article
|