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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
3
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pubmed:dateCreated |
1998-3-12
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pubmed:abstractText |
The spo0B gene, which exists as an operon with the obg gene, is required to initiate sporulation (stage 0) of Bacillus subtilis . This gene encodes a phosphotransferase in the multicomponent phosphorelay system. We here report the novel finding that a spo0B 5'-terminal SLR (stem-loop structure sequestering ribosome binding sequence; ACUCCUAA-X16-UUG GGAG U, Delta G = -8.71 kcal/mol) attenuated spo0B translation. The spo0B gene was efficiently transcribed but Spo0B protein was not overproduced in Escherichia coli when spo0B was induced using expression vectors carrying the SLR- spo0B region under control of the tac promoter. Deletion of the SLR from the vectors resulted in overexpression of spo0B . Therefore, to characterize expression of spo0B with a SLR in B.subtilis we constructed transcriptional and translational lacZ fusions combined with the spo0B 5'-terminal region with a deleted or mutagenized SLR. These constructs were subsequently introduced into B.subtilis as multiple and single copies, then beta-galactosidase activities were measured. The possible SLR also functioned as a negative cis element in B.subtilis. Furthermore, B.subtilis strain 1S16 (spo0B136) lysogenized straight phiCD0B-S and -W, harboring spo0B with mutagenized SLRs that were more (Delta G = -14.0 kcal/mol) and less-stable (Delta G = -1.31 kcal/mol) compared with the wild-type, exhibited null and wild-type sporulation respectively. These results indicate that the spo0B 5'-SLR affects spo0B gene expression for sporulation but that low expression of spo0B through the wild-type SLR was sufficient to initiate sporulation in B.subtilis.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/9443976-1370426,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9443976-1372983,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9443976-1375309,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9443976-1711026,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9443976-1846779,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9443976-1903544,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9443976-2106683,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9443976-2183291,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9443976-2449689,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9443976-2459711,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9443976-2496113,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9443976-2500423,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9443976-2537815,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9443976-271968,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9443976-2828193,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9443976-2985470,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9443976-3881765,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9443976-4215216,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9443976-4519026,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9443976-4598299,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9443976-5432063,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9443976-6163133,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9443976-6235151,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9443976-6260752,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9443976-6313226,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9443976-6330116,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9443976-6363212,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9443976-6402773,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9443976-6438629,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9443976-6769751,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9443976-6799842,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9443976-7541470,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9443976-7682278,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9443976-7961486,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9443976-8022289,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9443976-8282730,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9443976-8464402,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9443976-8479420
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Bacterial Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/GTP-Binding Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Obg GTP-binding protein, Bacteria,
http://linkedlifedata.com/resource/pubmed/chemical/RNA, Bacterial,
http://linkedlifedata.com/resource/pubmed/chemical/RNA, Messenger,
http://linkedlifedata.com/resource/pubmed/chemical/Recombinant Fusion Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Sigma Factor,
http://linkedlifedata.com/resource/pubmed/chemical/Transcription Factors,
http://linkedlifedata.com/resource/pubmed/chemical/spoIIR protein, Bacillus subtilis,
http://linkedlifedata.com/resource/pubmed/chemical/spore-specific proteins, Bacillus
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pubmed:status |
MEDLINE
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pubmed:month |
Feb
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pubmed:issn |
0305-1048
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
1
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pubmed:volume |
26
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
824-30
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:9443976-Amino Acid Sequence,
pubmed-meshheading:9443976-Bacillus subtilis,
pubmed-meshheading:9443976-Bacterial Proteins,
pubmed-meshheading:9443976-Base Sequence,
pubmed-meshheading:9443976-Escherichia coli,
pubmed-meshheading:9443976-GTP-Binding Proteins,
pubmed-meshheading:9443976-Gene Expression Regulation, Fungal,
pubmed-meshheading:9443976-Genes, Bacterial,
pubmed-meshheading:9443976-Molecular Sequence Data,
pubmed-meshheading:9443976-Nucleic Acid Conformation,
pubmed-meshheading:9443976-Operon,
pubmed-meshheading:9443976-Point Mutation,
pubmed-meshheading:9443976-Protein Biosynthesis,
pubmed-meshheading:9443976-RNA, Bacterial,
pubmed-meshheading:9443976-RNA, Messenger,
pubmed-meshheading:9443976-Recombinant Fusion Proteins,
pubmed-meshheading:9443976-Sequence Deletion,
pubmed-meshheading:9443976-Sigma Factor,
pubmed-meshheading:9443976-Spores, Bacterial,
pubmed-meshheading:9443976-Transcription Factors
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pubmed:year |
1998
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pubmed:articleTitle |
Translational attenuation of the Bacillus subtilis spo0B cistron by an RNA structure encompassing the initiation region.
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pubmed:affiliation |
Faculty of Agriculture, Tokyo University of Agriculture and Technology, Fuchu 183, Tokyo, Japan.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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