Linked Life Data

9441859

Source:http://linkedlifedata.com/resource/pubmed/id/9441859

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
6
pubmed:dateCreated
1998-3-23
pubmed:abstractText
Haemophilus somnus is able to survive and multiply in bovine blood monocytes (BBM) and alveolar macrophages (BAM), but the mechanisms used by H. somnus to evade killing mechanisms of bovine mononuclear phagocytes are not completely understood. To study the bactericidal ability of bovine mononuclear phagocytes following interaction with H. somnus, in vitro assay systems were developed to detect the luminol-dependent chemiluminescence response (LDCL) and nitric oxide (NO) production of BBM and BAM. Live logarithmically growing or stationary phase H. somnus inhibited the LDCL of BBM and BAM costimulated with opsonized Staphylococcus aureus. Inhibition of the LDCL response of BBM and BAM was not mediated by live H. somnus opsonized with hyperimmune serum, or by killed bacteria. H. somnus stimulated both BBM and BAM to produce NO at levels comparable with Escherichia coli lipopolysaccharide. While NO was being produced, viable H. somnus could still be isolated from the cell cultures. The ability of H. somnus to inhibit LDCL of both BBM and BAM, and resistance to NO killing may be an important mechanism that contributes to survival of the organism following ingestion by bovine mononuclear phagocytes.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Dec
pubmed:issn
0882-4010
pubmed:author
pubmed:copyrightInfo
Copyright 1997 Academic Press Limited.
pubmed:issnType
Print
pubmed:volume
23
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
327-33
pubmed:dateRevised
2011-11-17
pubmed:meshHeading
pubmed:year
1997
pubmed:articleTitle
Effect of Haemophilus somnus on nitric oxide production and chemiluminescence response of bovine blood monocytes and alveolar macrophages.
pubmed:affiliation
Veterinary Infectious Disease Organization, 120 Veterinary Road, Saskatoon, Saskatchewan, S7N 5E3, Canada.
pubmed:publicationType
Journal Article, In Vitro, Research Support, Non-U.S. Gov't