| pubmed-article:9441701 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:9441701 | lifeskim:mentions | umls-concept:C0086418 | lld:lifeskim |
| pubmed-article:9441701 | lifeskim:mentions | umls-concept:C0035322 | lld:lifeskim |
| pubmed-article:9441701 | lifeskim:mentions | umls-concept:C0079633 | lld:lifeskim |
| pubmed-article:9441701 | lifeskim:mentions | umls-concept:C0128897 | lld:lifeskim |
| pubmed-article:9441701 | lifeskim:mentions | umls-concept:C0162788 | lld:lifeskim |
| pubmed-article:9441701 | lifeskim:mentions | umls-concept:C0936012 | lld:lifeskim |
| pubmed-article:9441701 | pubmed:issue | 6 | lld:pubmed |
| pubmed-article:9441701 | pubmed:dateCreated | 1998-6-11 | lld:pubmed |
| pubmed-article:9441701 | pubmed:abstractText | Human retinal pigment epithelial (RPE) cells secrete chemokines, interleukin-8 (IL-8) and monocyte chemotactic protein-1 (MCP-1) in response to pro-inflammatory cytokines. In this study we (1) examined the efficiency of human RPE IL-8 and MCP-1 secretion, (2) determined the amount of neutrophil and monocyte chemotactic activity in human RPE cell conditioned media and cell extracts that is attributable to IL-8 and MCP-1, respectively, and (3) assessed the sensitivity of immunohistochemistry and in situ hybridization for detecting chemokine production by cytokine-stimulated human RPE cells. Conditioned media and extracts from human RPE cells stimulated with various physiologic concentrations of interleukin-1 beta (IL-1 beta) (0.2-20 ng ml-1), tumor necrosis factor (TNF-alpha) (0.2-20 ng ml-1) or interferon-gamma (IFN-gamma) (10-1000 U ml-1) were examined to compare secreted and cell associated levels of IL-8 and MCP-1 at various time points up to 24 hr. ELISA demonstrated that IL-8 and MCP-1 are both efficiently secreted by pro-inflammatory cytokine treated human RPE cells. Substantial dose- and time-dependent RPE secretion of IL-8 was observed following stimulation with IL-1 beta or TNF-alpha, but cell associated IL-8 was detectable only after high dose (20 ng ml-1) IL-1 beta stimulation and comprised less than 1% of the total IL-8 induced. Dose- and time-dependent RPE cell MCP-1 secretion was also observed following IL-1 beta > TNF-alpha > IFN-gamma stimulation, with an average of 4% of the total MCP-1 retained within RPE. Bioassays demonstrated neutrophil and monocyte chemotactic activity in conditioned media from stimulated RPE cells, but not in human RPE cell extracts. Inhibition of conditioned media-induced chemotaxis by specific anti-IL-8 or anti-MCP-1 antibodies demonstrated that IL-8 and MCP-1 were responsible for the majority of HRPE-derived neutrophil (> 60%) and monocyte (53-57%) chemotactic activity, respectively. Using in situ hybridization IL-8 mRNA was readily detected within IL-1 beta > TNF-alpha stimulated RPE cells and MCP-1 mRNA easily visualized within IL-1 beta > TNF-alpha > or IFN-gamma stimulated cells. Immunohistochemistry to detect IL-8 was positive only in RPE cells exposed to high dose IL-1 beta (20 ng ml-1) for 8 or 24 hr and was weak. Immunohistochemical staining for MCP-1 in RPE cells was more intense and was visualized within RPE cells stimulated with IL-beta, TNF-alpha, or IFN-gamma. This study demonstrates that: (1) RPE cells efficiently secrete IL-8 and MCP-1 upon stimulation with pro-inflammatory cytokines; (2) secreted IL-8 and MCP-1 account for the majority of human RPE neutrophil and monocyte chemotactic activity; (3) in situ hybridization readily detects IL-8 and MCP-1 mRNA in cytokine stimulated RPE cells; and (4) immunohistochemistry demonstrates cell-associated MCP-1 in cytokine stimulated RPE cells, but only minimal cell-associated IL-8. | lld:pubmed |
| pubmed-article:9441701 | pubmed:grant | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:9441701 | pubmed:grant | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:9441701 | pubmed:language | eng | lld:pubmed |
| pubmed-article:9441701 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:9441701 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:9441701 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:9441701 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:9441701 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:9441701 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:9441701 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:9441701 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:9441701 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:9441701 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:9441701 | pubmed:month | Dec | lld:pubmed |
| pubmed-article:9441701 | pubmed:issn | 0014-4835 | lld:pubmed |
| pubmed-article:9441701 | pubmed:author | pubmed-author:ElnerV MVM | lld:pubmed |
| pubmed-article:9441701 | pubmed:author | pubmed-author:KunkelS LSL | lld:pubmed |
| pubmed-article:9441701 | pubmed:author | pubmed-author:StrieterR MRM | lld:pubmed |
| pubmed-article:9441701 | pubmed:author | pubmed-author:ElnerS GSG | lld:pubmed |
| pubmed-article:9441701 | pubmed:author | pubmed-author:BurnstineM... | lld:pubmed |
| pubmed-article:9441701 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:9441701 | pubmed:volume | 65 | lld:pubmed |
| pubmed-article:9441701 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:9441701 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:9441701 | pubmed:pagination | 781-9 | lld:pubmed |
| pubmed-article:9441701 | pubmed:dateRevised | 2008-11-21 | lld:pubmed |
| pubmed-article:9441701 | pubmed:meshHeading | pubmed-meshheading:9441701-... | lld:pubmed |
| pubmed-article:9441701 | pubmed:meshHeading | pubmed-meshheading:9441701-... | lld:pubmed |
| pubmed-article:9441701 | pubmed:meshHeading | pubmed-meshheading:9441701-... | lld:pubmed |
| pubmed-article:9441701 | pubmed:meshHeading | pubmed-meshheading:9441701-... | lld:pubmed |
| pubmed-article:9441701 | pubmed:meshHeading | pubmed-meshheading:9441701-... | lld:pubmed |
| pubmed-article:9441701 | pubmed:meshHeading | pubmed-meshheading:9441701-... | lld:pubmed |
| pubmed-article:9441701 | pubmed:meshHeading | pubmed-meshheading:9441701-... | lld:pubmed |
| pubmed-article:9441701 | pubmed:meshHeading | pubmed-meshheading:9441701-... | lld:pubmed |
| pubmed-article:9441701 | pubmed:meshHeading | pubmed-meshheading:9441701-... | lld:pubmed |
| pubmed-article:9441701 | pubmed:meshHeading | pubmed-meshheading:9441701-... | lld:pubmed |
| pubmed-article:9441701 | pubmed:meshHeading | pubmed-meshheading:9441701-... | lld:pubmed |
| pubmed-article:9441701 | pubmed:meshHeading | pubmed-meshheading:9441701-... | lld:pubmed |
| pubmed-article:9441701 | pubmed:meshHeading | pubmed-meshheading:9441701-... | lld:pubmed |
| pubmed-article:9441701 | pubmed:meshHeading | pubmed-meshheading:9441701-... | lld:pubmed |
| pubmed-article:9441701 | pubmed:meshHeading | pubmed-meshheading:9441701-... | lld:pubmed |
| pubmed-article:9441701 | pubmed:meshHeading | pubmed-meshheading:9441701-... | lld:pubmed |
| pubmed-article:9441701 | pubmed:meshHeading | pubmed-meshheading:9441701-... | lld:pubmed |
| pubmed-article:9441701 | pubmed:meshHeading | pubmed-meshheading:9441701-... | lld:pubmed |
| pubmed-article:9441701 | pubmed:meshHeading | pubmed-meshheading:9441701-... | lld:pubmed |
| pubmed-article:9441701 | pubmed:year | 1997 | lld:pubmed |
| pubmed-article:9441701 | pubmed:articleTitle | Cell-associated human retinal pigment epithelium interleukin-8 and monocyte chemotactic protein-1: immunochemical and in-situ hybridization analyses. | lld:pubmed |
| pubmed-article:9441701 | pubmed:affiliation | Department of Ophthalmology (W. K. Kellogg Eye Center), University of Michigan, Ann Arbor 48105, USA. | lld:pubmed |
| pubmed-article:9441701 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:9441701 | pubmed:publicationType | Research Support, U.S. Gov't, P.H.S. | lld:pubmed |
| pubmed-article:9441701 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:9441701 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:9441701 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:9441701 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:9441701 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:9441701 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:9441701 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:9441701 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:9441701 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:9441701 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:9441701 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:9441701 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:9441701 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:9441701 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:9441701 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:9441701 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:9441701 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:9441701 | lld:pubmed |
