Linked Life Data

9441286

Source:http://linkedlifedata.com/resource/pubmed/id/9441286

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
6
pubmed:dateCreated
1998-1-20
pubmed:abstractText
The aim of the study was to evaluate the toxicity of the various anticoagulants against human clonogenic CD34+ cells. We have been evaluating the toxicity of: heparin (Gibco, USA), heparin (Polfa, Poland) ACD (acid citrate dextrose) solution and CPDA-1 (citrate phosphate dextrose adenine) solution. Using standard concentrations of these anticoagulants, we found that heparin (Gibco, USA) is not toxic to the human early haematopoietic cells. In comparison, the heparin from Polfa, which was found to be not toxic at standard concentrations (20 U/ml), inhibited almost completely the growth of CD34+ cells if used at higher concentrations (2000 U/ml). Interestingly, both ACD and CPDA-1 solutions were also found to possess moderate toxicity, which tends to increase with the prolongation of the exposure time. Therefore, the heparin from Gibco, being well tolerated by clonogenic CD34+ cells, should be recommended as an anticoagulant for harvesting viable haematopoietic cells for transplantation purposes, or for in vitro diagnostic tests.
pubmed:language
pol
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jun
pubmed:issn
0032-3772
pubmed:author
pubmed:issnType
Print
pubmed:volume
97
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
509-17
pubmed:dateRevised
2010-4-1
pubmed:meshHeading
pubmed:year
1997
pubmed:articleTitle
[The effect of selected anticoagulants on the clonogenicity of human hematopoietic progenitors. Transplantation implications].
pubmed:affiliation
Zak?adu Patologii Komórki Pomorskiej Akademii Medycznej.
pubmed:publicationType
Journal Article, English Abstract