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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
1
|
| pubmed:dateCreated |
1998-2-12
|
| pubmed:databankReference | |
| pubmed:abstractText |
A mouse cDNA encoding paired-box-containing gene (Pax-4) was cloned from a cDNA library of a mouse pancreatic islet beta cell line of MIN6. The predicted open reading frame encodes a protein of 349 amino acids with a calculated molecular weight (MW) of 38-kD. A human Pax-4 cDNA sequence encoding 350 amino acids was deduced from a human cosmid clone. The mouse nucleotide and deduced amino acid sequences exhibit 83.4 and 80.0% identity with those of deduced human Pax-4, respectively. Southern blot analysis suggested that the mouse Pax-4 gene exists as a single copy in the genome. Reverse transcription (RT)-PCR analysis suggested that the mouse Pax-4 gene is expressed in pancreatic islets, cultured islet beta cell lines of MIN6, beta TC, and NIT-1 cells, but not detectable in any of 13 adult mouse organs examined.
|
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical | |
| pubmed:status |
MEDLINE
|
| pubmed:month |
Jan
|
| pubmed:issn |
0006-291X
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:day |
6
|
| pubmed:volume |
242
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
176-80
|
| pubmed:dateRevised |
2006-11-15
|
| pubmed:meshHeading |
pubmed-meshheading:9439631-Amino Acid Sequence,
pubmed-meshheading:9439631-Animals,
pubmed-meshheading:9439631-Base Sequence,
pubmed-meshheading:9439631-Cell Line,
pubmed-meshheading:9439631-Cloning, Molecular,
pubmed-meshheading:9439631-Genes, Homeobox,
pubmed-meshheading:9439631-Homeodomain Proteins,
pubmed-meshheading:9439631-Humans,
pubmed-meshheading:9439631-Islets of Langerhans,
pubmed-meshheading:9439631-Mice,
pubmed-meshheading:9439631-Molecular Sequence Data,
pubmed-meshheading:9439631-Multigene Family,
pubmed-meshheading:9439631-Paired Box Transcription Factors,
pubmed-meshheading:9439631-Sequence Analysis, DNA,
pubmed-meshheading:9439631-Sequence Homology, Amino Acid,
pubmed-meshheading:9439631-Species Specificity,
pubmed-meshheading:9439631-Tissue Distribution,
pubmed-meshheading:9439631-Transcription Factors
|
| pubmed:year |
1998
|
| pubmed:articleTitle |
Molecular cloning of mouse paired-box-containing gene (Pax)-4 from an islet beta cell line and deduced sequence of human Pax-4.
|
| pubmed:affiliation |
Otsuka Department of Clinical and Molecular Nutrition, School of Medicine, University of Tokushima, Japan.
|
| pubmed:publicationType |
Journal Article,
Comparative Study,
Research Support, Non-U.S. Gov't
|