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rdf:type |
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lifeskim:mentions |
umls-concept:C0023884,
umls-concept:C0024487,
umls-concept:C0025914,
umls-concept:C0026809,
umls-concept:C0037473,
umls-concept:C0086045,
umls-concept:C0178719,
umls-concept:C0680730,
umls-concept:C1148554,
umls-concept:C1549542,
umls-concept:C1553628
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pubmed:issue |
1
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pubmed:dateCreated |
1998-3-3
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pubmed:abstractText |
A combination of 31P and 23Na NMR spectroscopy has been used to quantify the concentration of intracellular sodium, [Na]IC in the isolated and perfused mouse liver. The 31P resonances of dimethyl methylphosphonate and LaDOTP5-, markers of total tissue space and extracellular space, respectively, were used to determine the intracellular liver volume. For a mean wet weight of 1.7 +/- 0.3 g, the intracellular liver volume as measured by 31P NMR averaged 1.2 +/- 0.2 ml. The amount of intracellular sodium was measured from the baseline-resolved intracellular 23Na resonance during perfusion of the shift reagent, TmDOTP5-. These two measurements resulted in an NMR-determined value for [Na]IC of 29.0 +/- 5.2 mM. Separate measurement of total tissue Tm and Na by atomic absorption spectroscopy on the same samples provided an AAS-determined value for [Na]IC of 32.1 +/- 7.4 mM. These results indicate that intracellular sodium in the isolated, perfused liver is 100% visible by 23Na NMR spectroscopy.
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pubmed:grant |
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Jan
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pubmed:issn |
0740-3194
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
39
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
155-9
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pubmed:dateRevised |
2007-11-14
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pubmed:meshHeading |
pubmed-meshheading:9438449-Animals,
pubmed-meshheading:9438449-Biological Markers,
pubmed-meshheading:9438449-Disease Models, Animal,
pubmed-meshheading:9438449-Extracellular Space,
pubmed-meshheading:9438449-Intracellular Fluid,
pubmed-meshheading:9438449-Lanthanum,
pubmed-meshheading:9438449-Liver,
pubmed-meshheading:9438449-Magnetic Resonance Spectroscopy,
pubmed-meshheading:9438449-Male,
pubmed-meshheading:9438449-Mice,
pubmed-meshheading:9438449-Mice, Inbred Strains,
pubmed-meshheading:9438449-Organophosphorus Compounds,
pubmed-meshheading:9438449-Perfusion,
pubmed-meshheading:9438449-Phosphorus Isotopes,
pubmed-meshheading:9438449-Sensitivity and Specificity,
pubmed-meshheading:9438449-Sodium,
pubmed-meshheading:9438449-Sodium Isotopes,
pubmed-meshheading:9438449-Tissue Distribution
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pubmed:year |
1998
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pubmed:articleTitle |
Determination of the intracellular sodium concentration in perfused mouse liver by 31P and 23Na magnetic resonance spectroscopy.
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pubmed:affiliation |
Department of Chemistry, University of Texas at Dallas, Richardson, USA.
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pubmed:publicationType |
Journal Article,
Comparative Study,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
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