Linked Life Data

9437396

Source:http://linkedlifedata.com/resource/pubmed/id/9437396

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
1998-2-10
pubmed:abstractText
FACS analysis was used to determine the percent interferon (IFN)-gamma-, interleukin (IL)-4-, IL-10-, and CD30-positive T-cells extracted from adult periodontitis (AP) and healthy/gingivitis (H/G) subjects. Additionally, the percentages of IL-1 beta-, IL-10- and IL-12-producing B-cells and macrophages were ascertained. The percent IL-10+ CD8 cells extracted from AP lesions was decreased compared with H/G tissues (p = 0.033), and the percent CD30+ CD4 (p = 0.001) and CD30+ CD8 (p = 0.028) cells was higher in AP than in H/G tissues. The percentages of IL-1 beta + macrophages (p = 0.003) and IL-12+ B-cells (p = 0.034) were both higher in AP lesions than in H/G tissues. The specific effect of Porphyromonas gingivalis on the cytokine profiles of peripheral blood mononuclear cells isolated from P. gingivalis-infected AP and H/G patients was also determined. While there were no significant differences in the percent cytokine-positive T-cells after stimulation with P. gingivalis outer membrane antigens (OM) for 6 days compared with cells incubated in medium only, the percent CD30+ CD4 cells increased significantly (p = 0.047 and p = 0.063 for AP and H/G groups, respectively). There was also an increase in the percent IL-1 beta + B-cells from AP patients (p = 0.029), and the percent IL-12+ monocytes from AP and H/G subjects was higher than the percent IL-12+ B-cells, both after stimulation with P. gingivalis OM (p = 0.005 for the AP group and p = 0.058 and therefore not quite significant for the H/G group) and when incubated in medium alone (p = 0.016 and p = 0.015 for AP and H/G groups, respectively). This study has shown that IL-10+ CD8 cells may be significant in gingival lesions, and that CD30+ T-cells indicative of Th2 or Th0 cells may play a role in progressive periodontal disease. This study has also shown that B-cells produce IL-1 in the gingival lesion and that P. gingivalis may be significant in the induction of B-cell-induced IL-1.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
D
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jan
pubmed:issn
0022-0345
pubmed:author
pubmed:issnType
Print
pubmed:volume
77
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
16-26
pubmed:dateRevised
2008-11-21
pubmed:meshHeading
pubmed-meshheading:9437396-Adult, pubmed-meshheading:9437396-Antigens, CD30, pubmed-meshheading:9437396-B-Lymphocytes, pubmed-meshheading:9437396-Bacterial Outer Membrane Proteins, pubmed-meshheading:9437396-CD4-Positive T-Lymphocytes, pubmed-meshheading:9437396-Cytokines, pubmed-meshheading:9437396-Disease Progression, pubmed-meshheading:9437396-Female, pubmed-meshheading:9437396-Gingivitis, pubmed-meshheading:9437396-Humans, pubmed-meshheading:9437396-Interferon-gamma, pubmed-meshheading:9437396-Interleukin-1, pubmed-meshheading:9437396-Interleukin-10, pubmed-meshheading:9437396-Interleukin-12, pubmed-meshheading:9437396-Interleukin-4, pubmed-meshheading:9437396-Leukocytes, Mononuclear, pubmed-meshheading:9437396-Lymphocyte Activation, pubmed-meshheading:9437396-Macrophages, pubmed-meshheading:9437396-Male, pubmed-meshheading:9437396-Middle Aged, pubmed-meshheading:9437396-Periodontitis, pubmed-meshheading:9437396-Periodontium, pubmed-meshheading:9437396-Porphyromonas gingivalis, pubmed-meshheading:9437396-T-Lymphocytes, pubmed-meshheading:9437396-T-Lymphocytes, Helper-Inducer, pubmed-meshheading:9437396-Th2 Cells
pubmed:year
1998
pubmed:articleTitle
Cytokine profiles of cells extracted from humans with periodontal diseases.
pubmed:affiliation
Department of Dentistry, University of Queensland, Australia.
pubmed:publicationType
Journal Article, Comparative Study, Research Support, Non-U.S. Gov't