Linked Life Data

9435285

Source:http://linkedlifedata.com/resource/pubmed/id/9435285

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
4
pubmed:dateCreated
1998-4-2
pubmed:abstractText
The early stages of otic placode development depend on signals from neighbouring tissues including the hindbrain. The identity of these signals and of the responding placodal genes, however, is not known. We have identified a chick homeobox gene cNkx5-1, which is expressed in the otic placode beginning at stage 10 and exhibits a dynamic expression pattern during formation and further differentiation of the otic vesicle. In a series of heterotopic transplantation experiments, we demonstrate that cNkx5-1 can be activated in ectopic positions. However, significant differences in otic development and cNkx5-1 gene activity were observed when placodes were transplanted into the more rostral positions within the head mesenchyme or into the wing buds of older hosts. These results indicate that only the rostral tissues were able to induce and/or maintain ear development. Ectopically induced cNkx5-1 expression always reproduced the endogenous pattern within the lateral wall of the otocyst that is destined to form vestibular structures. In contrast, cPax2 which is expressed in the medial wall of the early otic vesicle later forming the cochlea never resumed its correct expression pattern after transplantation. Our experiments illustrate that only some aspects of gene expression and presumably pattern formation during inner ear development can be established and maintained ectopically. In particular, the dorsal vestibular structures seem to be programmed earlier and differently from the ventral cochlear part.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Feb
pubmed:issn
0950-1991
pubmed:author
pubmed:issnType
Print
pubmed:volume
125
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
645-54
pubmed:dateRevised
2007-11-15
pubmed:meshHeading
pubmed-meshheading:9435285-Animals, pubmed-meshheading:9435285-Base Sequence, pubmed-meshheading:9435285-Chick Embryo, pubmed-meshheading:9435285-Cochlea, pubmed-meshheading:9435285-DNA, Complementary, pubmed-meshheading:9435285-DNA-Binding Proteins, pubmed-meshheading:9435285-Ear, Inner, pubmed-meshheading:9435285-Gene Expression Regulation, Developmental, pubmed-meshheading:9435285-Genes, Homeobox, pubmed-meshheading:9435285-Genes, Regulator, pubmed-meshheading:9435285-Genetic Markers, pubmed-meshheading:9435285-Homeodomain Proteins, pubmed-meshheading:9435285-In Situ Hybridization, pubmed-meshheading:9435285-Mice, pubmed-meshheading:9435285-Molecular Sequence Data, pubmed-meshheading:9435285-Nerve Tissue Proteins, pubmed-meshheading:9435285-PAX2 Transcription Factor, pubmed-meshheading:9435285-Sequence Homology, Nucleic Acid, pubmed-meshheading:9435285-Signal Transduction, pubmed-meshheading:9435285-Transcription Factors, pubmed-meshheading:9435285-Transplantation, Heterotopic, pubmed-meshheading:9435285-Vestibule, Labyrinth, pubmed-meshheading:9435285-Wing
pubmed:year
1998
pubmed:articleTitle
Two regulatory genes, cNkx5-1 and cPax2, show different responses to local signals during otic placode and vesicle formation in the chick embryo.
pubmed:affiliation
Department of Cell and Molecular Biology, Technical University Braunschweig, Braunschweig, Germany.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't