Linked Life Data

9433899

Source:http://linkedlifedata.com/resource/pubmed/id/9433899

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
1998-2-19
pubmed:abstractText
The present study examined the effects of a short-distance triathlon on the induction of DNA effects in peripheral leukocytes, urinary excretion of oxidized DNA bases, and frequency of micronuclei in lymphocytes of human volunteers. Induction of DNA effects was measured as increased DNA migration using the alkaline comet assay. Increased DNA migration was found in leukocytes of all individuals at different time points after exercise and revealed a biphasic pattern. Twenty-four hours postexercise, elevated DNA migration was found, whereas lower values were detected 48 h after exercise. Seventy-two hours postexercise, the maximum increase in DNA migration was found and baseline values were still elevated after 120 h. A modified protocol of the comet assay for the detection of oxidized DNA bases revealed no differences in leukocytes before and directly after the triathlon. Urinary excretion of 8-hydroxy-2'-deoxyguanosine remained unaltered during the 5 consecutive days sampled. No differences were found in the micronucleus-frequency in lymphocytes before or 48 and 96 h after exercise. Our data suggest that DNA effects detected with the comet assay in leukocytes of humans after exercise are secondary effects that do not originate from oxidized DNA bases and do not result in chromosome damage.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jan
pubmed:issn
0891-5849
pubmed:author
pubmed:issnType
Print
pubmed:day
15
pubmed:volume
24
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
245-51
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed:year
1998
pubmed:articleTitle
Exercise-induced DNA effects in human leukocytes are not accompanied by increased formation of 8-hydroxy-2'-deoxyguanosine or induction of micronuclei.
pubmed:affiliation
Universität Ulm, Abteilung Medizinische Genetik, Germany.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't