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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
3
|
pubmed:dateCreated |
1998-2-2
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pubmed:abstractText |
Laser flash-induced photovoltage responses of the D85S and D85T mutants as well as of the wild-type acid blue form are similar and reflect intraprotein charge redistribution caused by retinal isomerization. The Cl- -induced transition of all of these blue forms into purple ones is accompanied by the appearance of electrogenic stages, which is probably associated with Cl- translocation in the cytoplasmic direction. Cl- translocation efficiency of these purple forms is much lower than that of the proton transport by the wild-type bacteriorhodopsin. The values of the efficiency do not exceed 15, 8 and 3% for the D85T, D85S and wild-type acid purple form, respectively. Cl- induces an additional electrogenic phase in the photovoltage responses of the D85S mutant and the wild-type acid purple form. This phase is supposed to be associated with the reversible Cl- movement in the extracellular direction. It is interesting that this component is absent in the photovoltage response of the D85T mutant which has, like halorhodopsin, a threonine residue at position 85.
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pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical | |
pubmed:status |
MEDLINE
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pubmed:month |
Dec
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pubmed:issn |
0014-5793
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pubmed:author | |
pubmed:issnType |
Print
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pubmed:day |
1
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pubmed:volume |
418
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
239-42
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:9428720-Bacteriorhodopsins,
pubmed-meshheading:9428720-Chlorides,
pubmed-meshheading:9428720-Halobacterium,
pubmed-meshheading:9428720-Ion Transport,
pubmed-meshheading:9428720-Lasers,
pubmed-meshheading:9428720-Mutation,
pubmed-meshheading:9428720-Photic Stimulation,
pubmed-meshheading:9428720-Purple Membrane
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pubmed:year |
1997
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pubmed:articleTitle |
Cl- -dependent photovoltage responses of bacteriorhodopsin: comparison of the D85T and D85S mutants and wild-type acid purple form.
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pubmed:affiliation |
Department of Photobiochemistry, A.N. Belozersky Institute of Physico-Chemical Biology, Moscow State University, Russia.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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