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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
1-2
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pubmed:dateCreated |
1998-1-22
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pubmed:databankReference |
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pubmed:abstractText |
Recently, we demonstrated a biphasic induction of the epithelial broad-spectrum matrix metalloproteinase (MMP) stromelysin-2 during cutaneous wound healing. Now we have generated a murine wound cDNA libary and have used it to isolate the putative cDNA of this murine matrix metalloproteinase. The predicted sequence of the protein shows 76 and 89% identity with its human and rat analogues, respectively. Stromelysin-2 and stromelysin-1 transcripts were both detected at very low levels in the lung and the heart of adult Balb/c mice, whereas stromelysin-2 mRNA expression alone was found at comparatively high levels in the small intestine, a tissue characterized by continuous epithelial renewal. Recombinant forms of murine stromelysin-1 and -2 produced in transfected COS cells were secreted and could be induced to undergo autocatalytic processing by addition of the organomercurial salt 4-aminophenylmercuric acetate (APMA).
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/4-aminophenylmercuriacetate,
http://linkedlifedata.com/resource/pubmed/chemical/DNA, Complementary,
http://linkedlifedata.com/resource/pubmed/chemical/Hemagglutinin Glycoproteins...,
http://linkedlifedata.com/resource/pubmed/chemical/Matrix Metalloproteinase 10,
http://linkedlifedata.com/resource/pubmed/chemical/Matrix Metalloproteinase 3,
http://linkedlifedata.com/resource/pubmed/chemical/Metalloendopeptidases,
http://linkedlifedata.com/resource/pubmed/chemical/Phenylmercuric Acetate,
http://linkedlifedata.com/resource/pubmed/chemical/RNA, Messenger,
http://linkedlifedata.com/resource/pubmed/chemical/Recombinant Fusion Proteins
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pubmed:status |
MEDLINE
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pubmed:month |
Nov
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pubmed:issn |
0378-1119
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
20
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pubmed:volume |
202
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
75-81
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:9427548-Amino Acid Sequence,
pubmed-meshheading:9427548-Animals,
pubmed-meshheading:9427548-Base Sequence,
pubmed-meshheading:9427548-COS Cells,
pubmed-meshheading:9427548-DNA, Complementary,
pubmed-meshheading:9427548-Female,
pubmed-meshheading:9427548-Gene Expression Regulation,
pubmed-meshheading:9427548-Hemagglutinin Glycoproteins, Influenza Virus,
pubmed-meshheading:9427548-Matrix Metalloproteinase 10,
pubmed-meshheading:9427548-Matrix Metalloproteinase 3,
pubmed-meshheading:9427548-Metalloendopeptidases,
pubmed-meshheading:9427548-Mice,
pubmed-meshheading:9427548-Mice, Inbred BALB C,
pubmed-meshheading:9427548-Molecular Sequence Data,
pubmed-meshheading:9427548-Open Reading Frames,
pubmed-meshheading:9427548-Organ Specificity,
pubmed-meshheading:9427548-Phenylmercuric Acetate,
pubmed-meshheading:9427548-RNA, Messenger,
pubmed-meshheading:9427548-Rats,
pubmed-meshheading:9427548-Recombinant Fusion Proteins,
pubmed-meshheading:9427548-Sequence Homology, Nucleic Acid
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pubmed:year |
1997
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pubmed:articleTitle |
cDNA cloning and expression of the gene encoding murine stromelysin-2 (MMP-10).
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pubmed:affiliation |
Max-Plank-Institue für Biochemie, Martinsried, Germany.
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pubmed:publicationType |
Journal Article,
Comparative Study,
Research Support, Non-U.S. Gov't
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