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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
3
|
| pubmed:dateCreated |
1998-2-3
|
| pubmed:abstractText |
The effects of granulocyte-macrophage colony-stimulating factor (GM-CSF), interleukin (IL)-3 and IL-6 on clonogenic growth of blast-cell progenitors from 19 immunologically defined CD10-positive B-lineage acute lymphoblastic leukemias (ALL) coexpressing (My+ALLs) or not (My-ALLs) myeloid antigens have been studied. Our results demonstrate that GM-CSF was able to support the clonogenic growth of blast cells from My+ALLs, being totally ineffective on My-All samples. Accordingly, both alpha and beta chains of GM-CSF receptor (R) were expressed by My+ALL blasts, as investigated by reverse-transcriptase polymerase chain reaction (RT-PCR). Colony cells from GM-CSF-stimulated My+ALL cultures displayed the same immunophenotype as primary leukemic cells at diagnosis (CD10+, CD19+, CD22+), and retained the expression of myeloid-associated antigens and of GM-CSF-R transcripts. Moreover, My+ALL blasts showed a preferential sensitivity to the growth-promoting activity of IL-3 and IL-6, as compared with My-ALL cells. In addition to rearrangements of the JH region of immunoglobulin genes, My+ALL cells showed aberrant rearrangements of gamma (three cases) and beta (two cases) T-cell receptor genes, as well as of bcr sequences (three cases). Our data, showing an unexpected cross-lineage response of My+ALLs to GM-CSF, and their preferential stimulation by IL-3 and IL-6, as compared with My-ALLs, further support the concept that My+ALLs represent a separate entity with unique biological features.
|
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Antigens, CD,
http://linkedlifedata.com/resource/pubmed/chemical/Granulocyte-Macrophage...,
http://linkedlifedata.com/resource/pubmed/chemical/Interleukins,
http://linkedlifedata.com/resource/pubmed/chemical/RNA, Messenger,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Granulocyte-Macrophage...
|
| pubmed:status |
MEDLINE
|
| pubmed:month |
Sep
|
| pubmed:issn |
1368-4736
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:volume |
3
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
141-51
|
| pubmed:dateRevised |
2007-11-15
|
| pubmed:meshHeading |
pubmed-meshheading:9426972-Adolescent,
pubmed-meshheading:9426972-Adult,
pubmed-meshheading:9426972-Aged,
pubmed-meshheading:9426972-Antigens, CD,
pubmed-meshheading:9426972-Burkitt Lymphoma,
pubmed-meshheading:9426972-Cell Division,
pubmed-meshheading:9426972-Child, Preschool,
pubmed-meshheading:9426972-Clone Cells,
pubmed-meshheading:9426972-Female,
pubmed-meshheading:9426972-Gene Rearrangement,
pubmed-meshheading:9426972-Granulocyte-Macrophage Colony-Stimulating Factor,
pubmed-meshheading:9426972-Humans,
pubmed-meshheading:9426972-Immunophenotyping,
pubmed-meshheading:9426972-Interleukins,
pubmed-meshheading:9426972-Male,
pubmed-meshheading:9426972-Middle Aged,
pubmed-meshheading:9426972-RNA, Messenger,
pubmed-meshheading:9426972-Receptors, Granulocyte-Macrophage Colony-Stimulating Factor,
pubmed-meshheading:9426972-Stem Cells
|
| pubmed:year |
1997
|
| pubmed:articleTitle |
Human granulocyte-macrophage colony-stimulating factor supports the clonogenic growth of B-lineage acute lymphoblastic leukemias expressing myeloid antigens.
|
| pubmed:affiliation |
Unità Operativa Leucemie e Trapianto di Midollo, IRCCS, Aviano, Italy. vgattei@ets.it
|
| pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
|