Linked Life Data

9426446

Source:http://linkedlifedata.com/resource/pubmed/id/9426446

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
1998-1-29
pubmed:abstractText
The dsRNA containing birnavirus, infectious pancreatic necrosis virus, possesses a virion-associated RNA-dependent RNA polymerase which acts both as primer and as polymerase during in vitro RNA synthesis (P. Dobos, 1995, Virology 208, 19-25). Using [alpha 32P]GTP, we have radiolabeled virion RNA in vitro and found that after deproteinization most of the labeled product comigrated in agarose gels with the 3-kbp viral genome, while the remainder migrated faster than the dsRNA and as a heterogeneous smear. Agarose gel electrophoresis (AGE) of denatured labeled virion RNA showed a radioactive smear ranging from approximately 100 nucleotides to up to 3000 nucleotide the size of genome length single stranded RNA. Hybridization experiments using strand-specific and end-specific obligonucleotides on Northern blots revealed that the radioactivity which migrates with the dsRNA during AGE represents small, 5' end plus RNA molecules of 100-500 nucleotides. The radioactivity in the faster migrating smear denotes incomplete dsRNAs where full-length, unlabeled minus strands are base-paired with labeled plus strands that are 3' truncated to different extents. This was confirmed by reverse transcription-polymerase chain reaction (RT-PCR) using end- and strand-specific obligonucleotide primers. The results indicated that 95% of incomplete dsRNA molecules consisted of full-length minus strands and 3' truncated plus strands. The implications of these findings are discussed in light of RNA replication mechanisms of dsRNA viruses belonging to other families.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Dec
pubmed:issn
0042-6822
pubmed:author
pubmed:issnType
Print
pubmed:day
8
pubmed:volume
239
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
62-70
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed:year
1997
pubmed:articleTitle
Incomplete dsRNA genomes in purified infectious pancreatic necrosis virus.
pubmed:affiliation
Department of Microbiology, College of Biological Science, University of Guelph, Ontario, Canada.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't