Linked Life Data

9426155

Source:http://linkedlifedata.com/resource/pubmed/id/9426155

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
1998-2-9
pubmed:abstractText
Treatment of reaggregate pituitary cell cultures of 14-day-old female rats with nerve growth factor (NGF) augmented the number of [3H]thymidine ([3H]T)-incorporating lactotrophs in a dose-dependent manner (0.03-3 nM). At least during short-term treatment NGF increased the total number of cells expressing prolactin (PRL) mRNA and enlarged the cytoplasmic area occupied by PRL mRNA but did not affect the number of cells and the cytoplasmic area containing PRL, suggesting that NGF recruits lactotrophs expressing PRL mRNA but not yet PRL. NGF also stimulated [3H]T incorporation in ACTH cells but not in somatotrophs, thyrotrophs and gonadotrophs. In addition, NGF augmented the total number of [3H]T-incorporating cells to a much higher extent than was expected from its effect on lactotrophs and ACTH cells, suggesting NGF also stimulates [3H]T-incorporation in non-hormone producing cells (progenitors or stem cells?). Around 40% of these [3H]T-incorporating cells in both control and NGF treated cultures showed immunoreactivity for the transcription factor Pit-1 in the nuclei, which is twice the percentage expected (18%) if these [3H]T-incorporating cells were the only known Pit-1 expressing cells in the pituitary i.e. lactotrophs, somatotrophs and thyrotrophs. The present data suggest that NGF has a mitogenic effect on several cell lineages in the pituitary: lactotrophs, corticotrophs and non-hormone-containing cells. The high proportion of mitotic non-hormone containing cells that express Pit-1 is consistent with the proposed role of Pit-1 in cell proliferation in the developing lactosomatotroph lineage.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Nov
pubmed:issn
0303-7207
pubmed:author
pubmed:issnType
Print
pubmed:day
15
pubmed:volume
134
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
119-27
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed:year
1997
pubmed:articleTitle
Mitogenic effects of nerve growth factor on different cell types in reaggregate cell cultures of immature rat pituitary.
pubmed:affiliation
Laboratory of Cell Pharmacology, University of Leuven, School of Medicine, Campus Gasthuisberg (O&N), Belgium.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't