| pubmed-article:9425632 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:9425632 | lifeskim:mentions | umls-concept:C0042071 | lld:lifeskim |
| pubmed-article:9425632 | lifeskim:mentions | umls-concept:C0033268 | lld:lifeskim |
| pubmed-article:9425632 | pubmed:issue | 3 | lld:pubmed |
| pubmed-article:9425632 | pubmed:dateCreated | 1998-2-18 | lld:pubmed |
| pubmed-article:9425632 | pubmed:abstractText | A system was developed to produce recombinant urokinase-type plasminogen activator in Escherichia coli. The urokinase-type plasminogen activator was produced with a 6 x His-tag at the C-terminus which was shown to have the same activity, after refolding, as the wild-type protein. Purification of the recombinant protein to homogeneity (95%) was possible by one-step affinity chromatography under denaturing conditions. As a result, proteolysis by bacterial proteases during purification was avoided. A higher refolding efficiency (40%) and a higher total recovery yield (25%) of the recombinant protein were obtained by this method. | lld:pubmed |
| pubmed-article:9425632 | pubmed:language | eng | lld:pubmed |
| pubmed-article:9425632 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:9425632 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:9425632 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:9425632 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:9425632 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:9425632 | pubmed:month | Dec | lld:pubmed |
| pubmed-article:9425632 | pubmed:issn | 1046-5928 | lld:pubmed |
| pubmed-article:9425632 | pubmed:author | pubmed-author:TaniYY | lld:pubmed |
| pubmed-article:9425632 | pubmed:author | pubmed-author:GurewichVV | lld:pubmed |
| pubmed-article:9425632 | pubmed:author | pubmed-author:PannellRR | lld:pubmed |
| pubmed-article:9425632 | pubmed:author | pubmed-author:GayM RMR | lld:pubmed |
| pubmed-article:9425632 | pubmed:author | pubmed-author:SunZ YZY | lld:pubmed |
| pubmed-article:9425632 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:9425632 | pubmed:volume | 11 | lld:pubmed |
| pubmed-article:9425632 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:9425632 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:9425632 | pubmed:pagination | 279-83 | lld:pubmed |
| pubmed-article:9425632 | pubmed:dateRevised | 2008-11-21 | lld:pubmed |
| pubmed-article:9425632 | pubmed:meshHeading | pubmed-meshheading:9425632-... | lld:pubmed |
| pubmed-article:9425632 | pubmed:meshHeading | pubmed-meshheading:9425632-... | lld:pubmed |
| pubmed-article:9425632 | pubmed:meshHeading | pubmed-meshheading:9425632-... | lld:pubmed |
| pubmed-article:9425632 | pubmed:meshHeading | pubmed-meshheading:9425632-... | lld:pubmed |
| pubmed-article:9425632 | pubmed:meshHeading | pubmed-meshheading:9425632-... | lld:pubmed |
| pubmed-article:9425632 | pubmed:meshHeading | pubmed-meshheading:9425632-... | lld:pubmed |
| pubmed-article:9425632 | pubmed:meshHeading | pubmed-meshheading:9425632-... | lld:pubmed |
| pubmed-article:9425632 | pubmed:meshHeading | pubmed-meshheading:9425632-... | lld:pubmed |
| pubmed-article:9425632 | pubmed:meshHeading | pubmed-meshheading:9425632-... | lld:pubmed |
| pubmed-article:9425632 | pubmed:meshHeading | pubmed-meshheading:9425632-... | lld:pubmed |
| pubmed-article:9425632 | pubmed:meshHeading | pubmed-meshheading:9425632-... | lld:pubmed |
| pubmed-article:9425632 | pubmed:meshHeading | pubmed-meshheading:9425632-... | lld:pubmed |
| pubmed-article:9425632 | pubmed:year | 1997 | lld:pubmed |
| pubmed-article:9425632 | pubmed:articleTitle | An efficient system for production of recombinant urokinase-type plasminogen activator. | lld:pubmed |
| pubmed-article:9425632 | pubmed:affiliation | Vascular Research Laboratory, Beth Israel-Deaconess Medical Center, Harvard Medical School, Boston, Massachusetts 02215, USA. | lld:pubmed |
| pubmed-article:9425632 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:9425632 | pubmed:publicationType | Comparative Study | lld:pubmed |
| pubmed-article:9425632 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
