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rdf:type |
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lifeskim:mentions |
umls-concept:C0017262,
umls-concept:C0033684,
umls-concept:C0035143,
umls-concept:C0080347,
umls-concept:C0086418,
umls-concept:C0162493,
umls-concept:C1171362,
umls-concept:C1257792,
umls-concept:C1416660,
umls-concept:C1514562,
umls-concept:C1515670,
umls-concept:C1549781,
umls-concept:C1880389,
umls-concept:C1883204,
umls-concept:C1883221
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pubmed:issue |
2
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pubmed:dateCreated |
1998-2-9
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pubmed:databankReference |
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pubmed:abstractText |
Members of the erythroid Krüppel-like factor (EKLF) multigene family contain three C-terminal zinc fingers, and they are typically expressed in a limited number of tissues. EKLF, the founding member, transactivates the beta-globin promoter by binding to the CACCC motif. EKLF is essential for expression of the beta-globin gene as demonstrated by gene deletion experiments in mice. Using a DNA probe from the zinc finger region of EKLF, we cloned a cDNA encoding a member of this family from a human vascular endothelial cell cDNA library. Sequence analysis indicated that our clone, hEZF, is the human homologue of the recently reported mouse EZF and GKLF. hEZF is a single-copy gene that maps to chromosome 9q31. By gel mobility shift analysis, purified recombinant hEZF protein bound specifically to a probe containing the CACCC core sequence. In co-transfection experiments, we found that sense but not antisense hEZF decreased the activity of a reporter plasmid containing the CACCC sequence upstream of the thymidine kinase promoter by 6-fold. In contrast, EKLF increased the activity of the reporter plasmid by 3-fold. By fusing hEZF to the DNA-binding domain of GAL4, we mapped a repression domain in hEZF to amino acids 181-388. We also found that amino acids 91-117 of hEZF confer an activation function on the GAL4 DNA-binding domain.
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pubmed:grant |
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Jan
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pubmed:issn |
0021-9258
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pubmed:author |
pubmed-author:AppW EWE,
pubmed-author:ChinM TMT,
pubmed-author:FoltaS CSC,
pubmed-author:HsiehC MCM,
pubmed-author:KINGD WDWJr,
pubmed-author:KUON MNM,
pubmed-author:LayneM DMD,
pubmed-author:McA'NultyM MMM,
pubmed-author:PerrellaM AMA,
pubmed-author:ThiN vN,
pubmed-author:WangHH,
pubmed-author:YoshizumiMM
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pubmed:issnType |
Print
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pubmed:day |
9
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pubmed:volume |
273
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
1026-31
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pubmed:dateRevised |
2008-11-21
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pubmed:meshHeading |
pubmed-meshheading:9422764-Amino Acid Sequence,
pubmed-meshheading:9422764-Animals,
pubmed-meshheading:9422764-Artificial Gene Fusion,
pubmed-meshheading:9422764-Cells, Cultured,
pubmed-meshheading:9422764-Chromosome Mapping,
pubmed-meshheading:9422764-Chromosomes, Human, Pair 9,
pubmed-meshheading:9422764-Cloning, Molecular,
pubmed-meshheading:9422764-DNA, Complementary,
pubmed-meshheading:9422764-DNA-Binding Proteins,
pubmed-meshheading:9422764-Endothelium, Vascular,
pubmed-meshheading:9422764-Humans,
pubmed-meshheading:9422764-Kruppel-Like Transcription Factors,
pubmed-meshheading:9422764-Mice,
pubmed-meshheading:9422764-Molecular Sequence Data,
pubmed-meshheading:9422764-Sequence Homology, Amino Acid,
pubmed-meshheading:9422764-Transcription Factors,
pubmed-meshheading:9422764-Transcriptional Activation,
pubmed-meshheading:9422764-Transfection,
pubmed-meshheading:9422764-Zinc Fingers
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pubmed:year |
1998
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pubmed:articleTitle |
Human EZF, a Krüppel-like zinc finger protein, is expressed in vascular endothelial cells and contains transcriptional activation and repression domains.
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pubmed:affiliation |
Cardiovascular Biology Laboratory, Harvard School of Public Health, Boston, Massachusetts 02115, USA.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
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