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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
1
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pubmed:dateCreated |
1998-1-30
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pubmed:abstractText |
Tracheal occlusion in utero is a potent stimulus of fetal lung growth. We describe the early growth mechanics of fetal lungs and type II pneumocytes after tracheal ligation (TL). Fetal rabbits underwent TL at 24 days gestational age (DGA; late pseudoglandular stage; term = 31 to 33 days) and were sacrificed at time intervals ranging from 1 to 5 days after TL. Lung growth was measured by stereological volumetry and bromodeoxyuridine (BrdU) pulse labeling. Pneumocyte II population kinetics were analyzed using a combination of anti-surfactant protein A and BrdU immunohistochemistry and computer-assisted morphometry. Nonoperated littermates served as controls. TL resulted in dramatically enhanced lung growth (lung weight/body weight was 5.00 +/- 0.81% in TL versus 2.52 +/- 0.13% in controls at 29 DGA; P < 0.001, unpaired Student's t-test). Post-TL lung growth was characterized by a 3-day lag-phase typified by relative stagnation of growth, followed by distension of airspaces, increased cell proliferation, and accelerated architectural and cellular maturation by postligation days 4 and 5. During the proliferation phase, the replicative activity of type II cells was markedly increased (type II cell BrdU labeling index was 10.0 +/- 4.1% in TL versus 1.1 +/- 0.3% for controls at 29 DGA; P < 0.02), but their numerical density decreased (3.0 +/- 0.5 x 10(-3)/microm2 in TL versus 4.5 +/- 0.3 x 10(-3)/microm2 in controls at 29 DGA; P < 0.02), suggesting accelerated terminal differentiation to type I cells. In conclusion, post-TL lung development is characterized by a well defined temporal pattern of lung growth and maturation. The rabbit model lends itself well to study the regulatory mechanisms underlying accelerated fetal lung growth after TL.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/9422535-14280143,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9422535-1996786,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9422535-3288247,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9422535-3757332,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9422535-4051314,
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http://linkedlifedata.com/resource/pubmed/commentcorrection/9422535-4294347,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9422535-5400911,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9422535-5803398,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9422535-6165237,
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http://linkedlifedata.com/resource/pubmed/commentcorrection/9422535-9044151
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
AIM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Jan
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pubmed:issn |
0002-9440
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
152
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
179-90
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:9422535-Animals,
pubmed-meshheading:9422535-Bromodeoxyuridine,
pubmed-meshheading:9422535-Constriction, Pathologic,
pubmed-meshheading:9422535-Embryonic and Fetal Development,
pubmed-meshheading:9422535-Fetal Diseases,
pubmed-meshheading:9422535-Fetus,
pubmed-meshheading:9422535-Lung,
pubmed-meshheading:9422535-Microscopy, Electron,
pubmed-meshheading:9422535-Proteolipids,
pubmed-meshheading:9422535-Pulmonary Surfactant-Associated Proteins,
pubmed-meshheading:9422535-Pulmonary Surfactants,
pubmed-meshheading:9422535-Rabbits,
pubmed-meshheading:9422535-Time Factors,
pubmed-meshheading:9422535-Tracheal Diseases
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pubmed:year |
1998
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pubmed:articleTitle |
Temporal pattern of accelerated lung growth after tracheal occlusion in the fetal rabbit.
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pubmed:affiliation |
Department of Pathology, Rhode Island Hospital and Brown University School of Medicine, Providence 02903, USA. 103107.2777@compuserve.com
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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