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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
7
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pubmed:dateCreated |
1998-1-22
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pubmed:abstractText |
The karyotype of H. annuus was analysed by computer-aided image processing with respect to the chromosome length, arm ratio, occurrence and chromosomal position of intercalary heterochromatin and the location of 18S/25S and 5S ribosomal RNA genes. The karyotype was subdivided into a group of four acrocentric chromosome pairs, of which two were distinguishable by HKG (HCl, KOH, Giemsa) banding and a group of 13 meta- to submetacentric pairs. The latter could be subdivided into seven pairs with one and six pairs with two HKG bands. Three pairs of submetacentric satellite chromosomes revealed 18S/25S rDNA loci after fluorescence in situ hybridization (FISH) and silver staining. A fourth, smaller and possibly inactive, locus occurred in the terminal position on a metacentric pair. One submetacentric satellite chromosome pair revealed a 5S rRNA gene locus in the pericentromeric position; a second locus marked a submetacentric pair with one HKG band. The C-banding technique marks exclusively centromeric heterochromatin. Measurements of chromosomes in combination with Giemsa banding and FISH enabled the discrimination of most chromosome pairs of the sunflower.
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pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Azure Stains,
http://linkedlifedata.com/resource/pubmed/chemical/Coloring Agents,
http://linkedlifedata.com/resource/pubmed/chemical/DNA, Ribosomal,
http://linkedlifedata.com/resource/pubmed/chemical/DNA Probes,
http://linkedlifedata.com/resource/pubmed/chemical/Feulgen stain,
http://linkedlifedata.com/resource/pubmed/chemical/Rosaniline Dyes
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pubmed:status |
MEDLINE
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pubmed:month |
Nov
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pubmed:issn |
0967-3849
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pubmed:author | |
pubmed:issnType |
Print
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pubmed:volume |
5
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
451-6
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:9421261-Azure Stains,
pubmed-meshheading:9421261-Chromosome Banding,
pubmed-meshheading:9421261-Chromosomes,
pubmed-meshheading:9421261-Coloring Agents,
pubmed-meshheading:9421261-DNA, Ribosomal,
pubmed-meshheading:9421261-DNA Probes,
pubmed-meshheading:9421261-Helianthus,
pubmed-meshheading:9421261-Image Processing, Computer-Assisted,
pubmed-meshheading:9421261-In Situ Hybridization, Fluorescence,
pubmed-meshheading:9421261-Karyotyping,
pubmed-meshheading:9421261-Rosaniline Dyes,
pubmed-meshheading:9421261-Silver Staining,
pubmed-meshheading:9421261-Staining and Labeling
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pubmed:year |
1997
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pubmed:articleTitle |
Karyotype analysis of Helianthus annuus using Giemsa banding and fluorescence in situ hybridization.
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pubmed:affiliation |
Institut für Züchtungsmethodik bei Gemüse der Bundesanstalt für Züchtungsforschung an Kulturpflanzen, Quedlinburg, Germany.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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