9421190

Source:http://linkedlifedata.com/resource/pubmed/id/9421190

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Predicate	Object
rdf:type	pubmed:Citation
lifeskim:mentions	umls-concept:C0007634, umls-concept:C0023828, umls-concept:C0026336, umls-concept:C1711411
pubmed:issue	2
pubmed:dateCreated	1998-1-15
pubmed:abstractText	The reliability of multi-shell dielectric models, used to describe the ac electrokinetic behaviour of cells, has been tested by performing electrorotation and dielectrophoretic measurements on unilamellar, oligolamellar, and multilamellar liposomes of diameters ranging from 5 to 24 microm. Fluorescence microscopy, flow cytometry and electron spin resonance were used to characterise the morphology and membranes of the liposomes. The dielectric properties of the various types of liposomes, based on appropriate dielectric shell models, were then analysed using a general purpose, recursive, algorithm. Through simulations, the confidence levels that can be assigned to parameters derived through application of simple shell models are estimated. From this, we confirm that electrorotation data enable accurate determinations to be made of the dielectric properties of the outermost membrane of liposomes, and provide good indications of the level of complexity of the shells and internal compartments. We also demonstrate that, used with sufficient additional information, such as that provided by dielectrophoresis, electrorotation data yields unique solutions for the dielectric parameters of liposome-like particles.
pubmed:grant	http://linkedlifedata.com/resource/pubmed/grant/R01 DK051065-05
pubmed:commentsCorrections	http://linkedlifedata.com/resource/pubmed/commentcorrection/9421190-1111634, http://linkedlifedata.com/resource/pubmed/commentcorrection/9421190-1631195, http://linkedlifedata.com/resource/pubmed/commentcorrection/9421190-290294, http://linkedlifedata.com/resource/pubmed/commentcorrection/9421190-3306721, http://linkedlifedata.com/resource/pubmed/commentcorrection/9421190-3719070, http://linkedlifedata.com/resource/pubmed/commentcorrection/9421190-573830, http://linkedlifedata.com/resource/pubmed/commentcorrection/9421190-5765779, http://linkedlifedata.com/resource/pubmed/commentcorrection/9421190-6854644, http://linkedlifedata.com/resource/pubmed/commentcorrection/9421190-7074191, http://linkedlifedata.com/resource/pubmed/commentcorrection/9421190-7846067, http://linkedlifedata.com/resource/pubmed/commentcorrection/9421190-8054355, http://linkedlifedata.com/resource/pubmed/commentcorrection/9421190-8318523, http://linkedlifedata.com/resource/pubmed/commentcorrection/9421190-8587932, http://linkedlifedata.com/resource/pubmed/commentcorrection/9421190-8652606
pubmed:language	eng
pubmed:journal	http://linkedlifedata.com/resource/pubmed/journal/0217513
pubmed:citationSubset	IM
pubmed:chemical	http://linkedlifedata.com/resource/pubmed/chemical/Liposomes
pubmed:status	MEDLINE
pubmed:month	Nov
pubmed:issn	0006-3002
pubmed:author	pubmed-author:BeckerF FFF, pubmed-author:ChanK LKL, pubmed-author:GascoyneP RPR, pubmed-author:PethigRR
pubmed:issnType	Print
pubmed:day	15
pubmed:volume	1349
pubmed:owner	NLM
pubmed:authorsComplete	Y
pubmed:pagination	182-96
pubmed:dateRevised	2009-11-18
pubmed:meshHeading	pubmed-meshheading:9421190-Electric Conductivity, pubmed-meshheading:9421190-Fluorescence, pubmed-meshheading:9421190-Liposomes, pubmed-meshheading:9421190-Rotation, pubmed-meshheading:9421190-Temperature
pubmed:year	1997
pubmed:articleTitle	Electrorotation of liposomes: verification of dielectric multi-shell model for cells.
pubmed:affiliation	Institute of Molecular and Biomolecular Electronics, University of Wales, Bangor, Gwynedd, UK.
pubmed:publicationType	Journal Article, Research Support, Non-U.S. Gov't