Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
1998-2-13
pubmed:abstractText
Initiation of translation of hepatitis C virus and classical swine fever virus mRNAs results from internal ribosomal entry. We reconstituted internal ribosomal entry in vitro from purified translation components and monitored assembly of 48S ribosomal preinitiation complexes by toe-printing. Ribosomal subunits (40S) formed stable binary complexes on both mRNAs. The complex structure of these RNAs determined the correct positioning of the initiation codon in the ribosomal "P" site in binary complexes. Ribosomal binding and positioning on these mRNAs did not require the initiation factors eIF3, eIF4A, eIF4B, and eIF4F and translation of these mRNAs was not inhibited by a trans-dominant eIF4A mutant. Addition of Met-tRNAiMet, eIF2, and GTP to these binary ribosomal complexes resulted in formation of 48S preinitiation complexes. The striking similarities between this eukaryotic initiation mechanism and the mechanism of translation initiation in prokaryotes are discussed.
pubmed:grant
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/9420332-1214295, http://linkedlifedata.com/resource/pubmed/commentcorrection/9420332-1310072, http://linkedlifedata.com/resource/pubmed/commentcorrection/9420332-1310759, http://linkedlifedata.com/resource/pubmed/commentcorrection/9420332-1316679, http://linkedlifedata.com/resource/pubmed/commentcorrection/9420332-1329037, http://linkedlifedata.com/resource/pubmed/commentcorrection/9420332-1620067, http://linkedlifedata.com/resource/pubmed/commentcorrection/9420332-1656091, http://linkedlifedata.com/resource/pubmed/commentcorrection/9420332-1730701, http://linkedlifedata.com/resource/pubmed/commentcorrection/9420332-1849266, http://linkedlifedata.com/resource/pubmed/commentcorrection/9420332-2002510, http://linkedlifedata.com/resource/pubmed/commentcorrection/9420332-2170120, http://linkedlifedata.com/resource/pubmed/commentcorrection/9420332-2184885, http://linkedlifedata.com/resource/pubmed/commentcorrection/9420332-2298214, http://linkedlifedata.com/resource/pubmed/commentcorrection/9420332-2468068, http://linkedlifedata.com/resource/pubmed/commentcorrection/9420332-2668952, http://linkedlifedata.com/resource/pubmed/commentcorrection/9420332-2763466, http://linkedlifedata.com/resource/pubmed/commentcorrection/9420332-3683388, http://linkedlifedata.com/resource/pubmed/commentcorrection/9420332-459891, http://linkedlifedata.com/resource/pubmed/commentcorrection/9420332-5339543, http://linkedlifedata.com/resource/pubmed/commentcorrection/9420332-592399, http://linkedlifedata.com/resource/pubmed/commentcorrection/9420332-6196023, http://linkedlifedata.com/resource/pubmed/commentcorrection/9420332-641055, http://linkedlifedata.com/resource/pubmed/commentcorrection/9420332-641056, http://linkedlifedata.com/resource/pubmed/commentcorrection/9420332-6853548, http://linkedlifedata.com/resource/pubmed/commentcorrection/9420332-7489514, http://linkedlifedata.com/resource/pubmed/commentcorrection/9420332-7559430, http://linkedlifedata.com/resource/pubmed/commentcorrection/9420332-7781762, http://linkedlifedata.com/resource/pubmed/commentcorrection/9420332-7809946, http://linkedlifedata.com/resource/pubmed/commentcorrection/9420332-7831836, http://linkedlifedata.com/resource/pubmed/commentcorrection/9420332-7941341, http://linkedlifedata.com/resource/pubmed/commentcorrection/9420332-8064856, http://linkedlifedata.com/resource/pubmed/commentcorrection/9420332-8131750, http://linkedlifedata.com/resource/pubmed/commentcorrection/9420332-8318536, http://linkedlifedata.com/resource/pubmed/commentcorrection/9420332-8503895, http://linkedlifedata.com/resource/pubmed/commentcorrection/9420332-8595564, http://linkedlifedata.com/resource/pubmed/commentcorrection/9420332-8643645, http://linkedlifedata.com/resource/pubmed/commentcorrection/9420332-8809014, http://linkedlifedata.com/resource/pubmed/commentcorrection/9420332-8846793, http://linkedlifedata.com/resource/pubmed/commentcorrection/9420332-8849773, http://linkedlifedata.com/resource/pubmed/commentcorrection/9420332-8941321, http://linkedlifedata.com/resource/pubmed/commentcorrection/9420332-8943341, http://linkedlifedata.com/resource/pubmed/commentcorrection/9420332-8943342, http://linkedlifedata.com/resource/pubmed/commentcorrection/9420332-8972770, http://linkedlifedata.com/resource/pubmed/commentcorrection/9420332-8985370
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
http://linkedlifedata.com/resource/pubmed/chemical/Codon, Initiator, http://linkedlifedata.com/resource/pubmed/chemical/DNA Primers, http://linkedlifedata.com/resource/pubmed/chemical/Eukaryotic Initiation Factor-2, http://linkedlifedata.com/resource/pubmed/chemical/Eukaryotic Initiation Factor-3, http://linkedlifedata.com/resource/pubmed/chemical/Eukaryotic Initiation Factor-4A, http://linkedlifedata.com/resource/pubmed/chemical/Eukaryotic Initiation Factor-4F, http://linkedlifedata.com/resource/pubmed/chemical/Eukaryotic Initiation Factors, http://linkedlifedata.com/resource/pubmed/chemical/Peptide Initiation Factors, http://linkedlifedata.com/resource/pubmed/chemical/RNA, Transfer, Met, http://linkedlifedata.com/resource/pubmed/chemical/RNA, Viral, http://linkedlifedata.com/resource/pubmed/chemical/Ribosomal Proteins, http://linkedlifedata.com/resource/pubmed/chemical/eIF-4B, http://linkedlifedata.com/resource/pubmed/chemical/ribosomal protein S9
pubmed:status
MEDLINE
pubmed:month
Jan
pubmed:issn
0890-9369
pubmed:author
pubmed:issnType
Print
pubmed:day
1
pubmed:volume
12
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
67-83
pubmed:dateRevised
2009-11-18
pubmed:meshHeading
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