Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
1998-1-28
pubmed:abstractText
The Epstein-Barr virus gH-gL complex includes a third glycoprotein, gp42, which is the product of the BZLF2 open reading frame (ORF). gp42 has been implicated as critical to infection of the B lymphocyte by virtue of its interaction with HLA class II on the B-cell surface. A neutralizing antibody that reacts with gp42 inhibits virus-cell fusion and blocks binding of gp42 to HLA class II; antibody to HLA class II can inhibit infection, and B cells that lack HLA class II can only be infected if HLA class II expression is restored. To confirm whether gp42 is an essential component of the virion, we derived a recombinant virus with a selectable marker inserted into the BZLF2 ORF to interrupt expression of the protein. A complex of gH and gL was expressed by the recombinant virus in the absence of gp42. Recombinant virus egressed from the cell normally and could bind to receptor-positive cells. It had, however, lost the ability to infect or transform B lymphocytes. Treatment with polyethylene glycol restored the infectivity of recombinant virus, confirming that gp42 is essential for penetration of the B-cell membrane.
pubmed:grant
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/9420211-1309250, http://linkedlifedata.com/resource/pubmed/commentcorrection/9420211-1309917, http://linkedlifedata.com/resource/pubmed/commentcorrection/9420211-1312629, http://linkedlifedata.com/resource/pubmed/commentcorrection/9420211-1316488, http://linkedlifedata.com/resource/pubmed/commentcorrection/9420211-1328481, http://linkedlifedata.com/resource/pubmed/commentcorrection/9420211-1647567, http://linkedlifedata.com/resource/pubmed/commentcorrection/9420211-2540161, http://linkedlifedata.com/resource/pubmed/commentcorrection/9420211-2545914, http://linkedlifedata.com/resource/pubmed/commentcorrection/9420211-2831372, http://linkedlifedata.com/resource/pubmed/commentcorrection/9420211-2831373, http://linkedlifedata.com/resource/pubmed/commentcorrection/9420211-2836619, http://linkedlifedata.com/resource/pubmed/commentcorrection/9420211-2838568, http://linkedlifedata.com/resource/pubmed/commentcorrection/9420211-3001337, http://linkedlifedata.com/resource/pubmed/commentcorrection/9420211-3016991, http://linkedlifedata.com/resource/pubmed/commentcorrection/9420211-3029986, http://linkedlifedata.com/resource/pubmed/commentcorrection/9420211-3156185, http://linkedlifedata.com/resource/pubmed/commentcorrection/9420211-6087149, http://linkedlifedata.com/resource/pubmed/commentcorrection/9420211-6177865, http://linkedlifedata.com/resource/pubmed/commentcorrection/9420211-6248876, http://linkedlifedata.com/resource/pubmed/commentcorrection/9420211-6263973, http://linkedlifedata.com/resource/pubmed/commentcorrection/9420211-6276755, http://linkedlifedata.com/resource/pubmed/commentcorrection/9420211-6319296, http://linkedlifedata.com/resource/pubmed/commentcorrection/9420211-7510086, http://linkedlifedata.com/resource/pubmed/commentcorrection/9420211-7539502, http://linkedlifedata.com/resource/pubmed/commentcorrection/9420211-7618278, http://linkedlifedata.com/resource/pubmed/commentcorrection/9420211-7692666, http://linkedlifedata.com/resource/pubmed/commentcorrection/9420211-7747461, http://linkedlifedata.com/resource/pubmed/commentcorrection/9420211-7783207, http://linkedlifedata.com/resource/pubmed/commentcorrection/9420211-7817877, http://linkedlifedata.com/resource/pubmed/commentcorrection/9420211-7933147, http://linkedlifedata.com/resource/pubmed/commentcorrection/9420211-8057484, http://linkedlifedata.com/resource/pubmed/commentcorrection/9420211-8383241, http://linkedlifedata.com/resource/pubmed/commentcorrection/9420211-8384759, http://linkedlifedata.com/resource/pubmed/commentcorrection/9420211-8393232, http://linkedlifedata.com/resource/pubmed/commentcorrection/9420211-8397282, http://linkedlifedata.com/resource/pubmed/commentcorrection/9420211-8421898, http://linkedlifedata.com/resource/pubmed/commentcorrection/9420211-8764069, http://linkedlifedata.com/resource/pubmed/commentcorrection/9420211-8794379, http://linkedlifedata.com/resource/pubmed/commentcorrection/9420211-9151859, http://linkedlifedata.com/resource/pubmed/commentcorrection/9420211-9188610
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jan
pubmed:issn
0022-538X
pubmed:author
pubmed:issnType
Print
pubmed:volume
72
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
158-63
pubmed:dateRevised
2009-11-18
pubmed:meshHeading
pubmed:year
1998
pubmed:articleTitle
Epstein-Barr virus lacking glycoprotein gp42 can bind to B cells but is not able to infect.
pubmed:affiliation
School of Biological Sciences, University of Missouri-Kansas City, 64110, USA.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S.