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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
5-6
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pubmed:dateCreated |
1998-2-25
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pubmed:abstractText |
A single-chain Fv (sFv) was expressed from the variable regions of the CD40-specific mAb G28-5. The molecule bound CD40 with a high affinity (2.2 nM) and was a monomer in solution. Surprisingly, G28-5 sFv was a potent CD40 agonist that rapidly crosslinked CD40 on the cell surface but did not crosslink CD40-Ig in solution. G28-5 sFv was a more potent agonist than G28-5 IgG and was able to stimulate CD40 responses by B cells and monocytes. G28-5 IgG partially blocked, whereas G28-5 sFv augmented CD40 responses during stimulation with natural ligand (gp39-CD8 fusion protein). These results indicate that the functional activity of ligands built from the binding site of G28-5 is highly dependent upon the size and physical properties of the molecule both in solution and on the cell surfaces.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:issn |
1040-8401
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pubmed:author |
pubmed-author:AruffoAA,
pubmed-author:BrownT JTJ,
pubmed-author:FranciscoJ AJA,
pubmed-author:GillilandL KLK,
pubmed-author:GordonM LML,
pubmed-author:GrosmaireL SLS,
pubmed-author:HollenbaughDD,
pubmed-author:KieferH AHA,
pubmed-author:LedbetterJ AJA,
pubmed-author:Mischel-PettyNN,
pubmed-author:MittlerR SRS,
pubmed-author:Moran-DavisPP,
pubmed-author:NadlerS GSG,
pubmed-author:SiadakA WAW,
pubmed-author:SiegallC BCB
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pubmed:issnType |
Print
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pubmed:volume |
17
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
427-35
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pubmed:dateRevised |
2005-11-16
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pubmed:meshHeading |
pubmed-meshheading:9419430-Animals,
pubmed-meshheading:9419430-Antibodies, Monoclonal,
pubmed-meshheading:9419430-Antigens, CD40,
pubmed-meshheading:9419430-B-Lymphocytes,
pubmed-meshheading:9419430-Cells, Cultured,
pubmed-meshheading:9419430-Cloning, Molecular,
pubmed-meshheading:9419430-Endothelium,
pubmed-meshheading:9419430-Gene Expression,
pubmed-meshheading:9419430-Humans,
pubmed-meshheading:9419430-Immunoglobulin Fragments,
pubmed-meshheading:9419430-Immunoglobulin Variable Region,
pubmed-meshheading:9419430-Monocytes,
pubmed-meshheading:9419430-NF-kappa B
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pubmed:year |
1997
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pubmed:articleTitle |
Agonistic activity of a CD40-specific single-chain Fv constructed from the variable regions of mAb G28-5.
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pubmed:affiliation |
Bristol-Myers Squibb Pharmaceutical Research Institute, Seattle, WA 98121, USA.
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pubmed:publicationType |
Journal Article,
Review
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