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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
1
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pubmed:dateCreated |
1998-2-3
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pubmed:abstractText |
The murine Htf9-a/RanBP1 and Htf9-c genes are divergently transcribed from a shared TATA-less promoter. Transcription of both genes is initiated on complementary DNA strands and is controlled by cell cycle-dependent mechanisms. The bidirectional promoter harbors a genomic footprint flanking the major transcription start site of both genes. Transient promoter assays showed that the footprinted element is important for transcription of both genes. Protein-binding experiments and antibody assays indicated that members of the retinoid X receptor family interact with the double-stranded site. In addition, distinct factors interact with single DNA strands of the element. Double-stranded binding factors were highly expressed in liver cells, in which neither gene is transcribed, while single-stranded binding proteins were abundant in cycling cells, in which transcription of both genes is efficient. In vivo S1 analysis of the promoter depicted an S1-sensitive organization in cells in which transcription of both genes is active; S1 sensitivity was not detected in conditions of transcriptional repression. Thus, the same element is a target for either retinoid X receptor factors, or for single-stranded binding proteins, and form distinct complexes in different cellular conditions depending on the DNA conformation in the binding site.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Cell Extracts,
http://linkedlifedata.com/resource/pubmed/chemical/DNA, Single-Stranded,
http://linkedlifedata.com/resource/pubmed/chemical/DNA-Binding Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/GTP-Binding Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Htf9c protein, mouse,
http://linkedlifedata.com/resource/pubmed/chemical/Nuclear Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Retinoic Acid,
http://linkedlifedata.com/resource/pubmed/chemical/Retinoid X Receptors,
http://linkedlifedata.com/resource/pubmed/chemical/Transcription Factors,
http://linkedlifedata.com/resource/pubmed/chemical/ran GTP-Binding Protein,
http://linkedlifedata.com/resource/pubmed/chemical/ran-binding protein 1
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pubmed:status |
MEDLINE
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pubmed:month |
Jan
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pubmed:issn |
0021-9258
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
2
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pubmed:volume |
273
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
495-505
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pubmed:dateRevised |
2008-11-21
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pubmed:meshHeading |
pubmed-meshheading:9417108-3T3 Cells,
pubmed-meshheading:9417108-Animals,
pubmed-meshheading:9417108-Base Sequence,
pubmed-meshheading:9417108-Binding Sites,
pubmed-meshheading:9417108-Cell Extracts,
pubmed-meshheading:9417108-DNA, Single-Stranded,
pubmed-meshheading:9417108-DNA Footprinting,
pubmed-meshheading:9417108-DNA-Binding Proteins,
pubmed-meshheading:9417108-Fibroblasts,
pubmed-meshheading:9417108-GTP-Binding Proteins,
pubmed-meshheading:9417108-Liver,
pubmed-meshheading:9417108-Mice,
pubmed-meshheading:9417108-Molecular Sequence Data,
pubmed-meshheading:9417108-Mutation,
pubmed-meshheading:9417108-Nuclear Proteins,
pubmed-meshheading:9417108-Promoter Regions, Genetic,
pubmed-meshheading:9417108-Proteins,
pubmed-meshheading:9417108-Receptors, Retinoic Acid,
pubmed-meshheading:9417108-Retinoid X Receptors,
pubmed-meshheading:9417108-Transcription Factors,
pubmed-meshheading:9417108-Transcriptional Activation,
pubmed-meshheading:9417108-ran GTP-Binding Protein
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pubmed:year |
1998
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pubmed:articleTitle |
Interactions with single-stranded and double-stranded DNA-binding factors and alternative promoter conformation upon transcriptional activation of the Htf9-a/RanBP1 and Htf9-c genes.
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pubmed:affiliation |
CNR Centre of Evolutionary Genetics, c/o Department of Genetics and Molecular Biology, University "La Sapienza," Rome 00185, Italy.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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