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rdf:type | |
lifeskim:mentions | |
pubmed:dateCreated |
1998-2-26
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pubmed:abstractText |
Brain tissue of rats pretreated with methylprednisolone or with the 21-aminosteroid U74389F, and that of untreated control rats, was assessed for the expression of Annexin-1 (Anx-1) and the transcription of its mRNA. For this purpose Anx-1 cDNA was amplified and simultaneously a T7-RNA-polymerase promotor was incorporated into the cDNA using Polymerase Chain Reaction (PCR). Then digoxigenin-11-UTP was incorporated into the transcribed cRNA with T7-RNA-polymerase. With this probe in situ hybridization was carried out in sections of the brain. The probe was visualized by an immunoassay using an anti-digoxigenin antibody conjugate. Anx-1 protein was assessed by means of immunohistochemistry using a polyclonal antibody. The various brain areas of the control animals showed an appreciable amount of Anx-1 at mRNA or protein level; on the other hand, the animals which had been pretreated with either steroid, showed a more intense Anx-1 mRNA signal than the controls in many areas. In the pretreated animals Anx-1 immunostaining was unchanged in cortex, basal ganglia, amygdala and septum, but more intense in hippocampus, hypothalamus and thalamus. In ependyma, choroid plexus, meninges, and vascular walls there was no Anx-1 mRNA transcription detectable. An opposite profile was shown by the Anx-1 immunoreactivity, the protein was present in control animals as well as the steroid-pretreated animals, suggesting that here the protein was either from systemic origin, or has diffused from adjacent structures. The results indicate that Anx-1 mRNA transcription is upregulated by either steroid, and that in the untreated animals there is a resting level of Anx-1 mRNA transcription, presumably reflecting physiological influences on Anx-1 expression.
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pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Annexin A1,
http://linkedlifedata.com/resource/pubmed/chemical/Methylprednisolone,
http://linkedlifedata.com/resource/pubmed/chemical/Pregnatrienes,
http://linkedlifedata.com/resource/pubmed/chemical/RNA, Messenger,
http://linkedlifedata.com/resource/pubmed/chemical/U 74389F
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pubmed:status |
MEDLINE
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pubmed:issn |
0065-1419
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pubmed:author | |
pubmed:issnType |
Print
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pubmed:volume |
70
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
222-4
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pubmed:dateRevised |
2004-11-17
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pubmed:meshHeading |
pubmed-meshheading:9416328-Animals,
pubmed-meshheading:9416328-Annexin A1,
pubmed-meshheading:9416328-Brain,
pubmed-meshheading:9416328-Immunohistochemistry,
pubmed-meshheading:9416328-In Situ Hybridization,
pubmed-meshheading:9416328-Methylprednisolone,
pubmed-meshheading:9416328-Polymerase Chain Reaction,
pubmed-meshheading:9416328-Pregnatrienes,
pubmed-meshheading:9416328-RNA, Messenger,
pubmed-meshheading:9416328-Rats
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pubmed:year |
1997
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pubmed:articleTitle |
Expression of annexin and annexin-mRNA in rat brain under influence of steroid drugs.
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pubmed:affiliation |
Department of Neurosurgery, University Hospital Groningen, The Netherlands.
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pubmed:publicationType |
Journal Article
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