Linked Life Data

9414663

Source:http://linkedlifedata.com/resource/pubmed/id/9414663

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
10
pubmed:dateCreated
1998-1-15
pubmed:abstractText
In 5-day incubation of an estrogen receptor-negative human ovarian cancer cell line (KF) with N,N-diethyl-2-[4-(phenylmethyl)phenoxy]ethanamine-HCl (DPPE), the concentration of DPPE required for 50% inhibition of KF cell proliferation (IC50) was 1.7 microM. The IC50 of DPPE for inhibition of protein kinase C (PKC) activity was 3.0 microM, a similar value to those of other antiestrogens such as tamoxifen and clomiphene. DPPE also inhibited phosphorylation of mitogen-activated protein kinase in KF cells. When treatment with DPPE was started 7 days after inoculation of KF cells into nude mice, 50 mg/kg DPPE alone resulted in a significant growth retardation in the early stage of tumor growth. Although 25 mg/kg DPPE showed a similar effect to 2 mg/kg cisplatin (CDDP), the combination had the most marked tumor growth-inhibitory effect. Nude mice treated with combinations of CDDP and DPPE survived significantly longer than not only untreated, but also CDDP-alone-treated mice, while 50 mg/kg but not 25 mg/kg DPPE alone had an effect comparable to that of 2 mg/kg CDDP alone. If treatment with DPPE was begun from the day after tumor inoculation, the inhibitory effect of DPPE was further enhanced, especially when combined with CDDP. If treatment with DPPE was started in nude mice with a lower tumor burden, 25 mg/kg as well as 50 mg/kg DPPE had a similar effect to 2 mg/kg CDDP, in terms of survival. When DPPE was combined with CDDP, the effect was significantly enhanced, compared to that of either alone. These treatments could be done without any adverse side effect. Thus, we conclude that DPPE has an antiestrogen action and its tumor growth-inhibiting activity is enhanced on administration in combination with CDDP.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Oct
pubmed:issn
0910-5050
pubmed:author
pubmed:issnType
Print
pubmed:volume
88
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
1003-8
pubmed:dateRevised
2007-11-15
pubmed:meshHeading
pubmed-meshheading:9414663-Animals, pubmed-meshheading:9414663-Antineoplastic Agents, pubmed-meshheading:9414663-Calcium-Calmodulin-Dependent Protein Kinases, pubmed-meshheading:9414663-Cell Division, pubmed-meshheading:9414663-Cisplatin, pubmed-meshheading:9414663-Clomiphene, pubmed-meshheading:9414663-Drug Combinations, pubmed-meshheading:9414663-Female, pubmed-meshheading:9414663-Humans, pubmed-meshheading:9414663-Mice, pubmed-meshheading:9414663-Mice, Nude, pubmed-meshheading:9414663-Neoplasm Transplantation, pubmed-meshheading:9414663-Ovarian Neoplasms, pubmed-meshheading:9414663-Phenyl Ethers, pubmed-meshheading:9414663-Phosphorylation, pubmed-meshheading:9414663-Protein Kinase C, pubmed-meshheading:9414663-Survival Rate, pubmed-meshheading:9414663-Tamoxifen, pubmed-meshheading:9414663-Tumor Cells, Cultured
pubmed:year
1997
pubmed:articleTitle
Growth-inhibitory effects of N,N-diethyl-2-[4-(phenylmethyl)phenoxy]-ethanamine-HCl combined with cisplatin on human ovarian cancer cells inoculated into nude mice.
pubmed:affiliation
Department of Obstetrics and Gynecology, National Defense Medical College, Tokorozawa.
pubmed:publicationType
Journal Article