Linked Life Data

9414547

Source:http://linkedlifedata.com/resource/pubmed/id/9414547

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
4
pubmed:dateCreated
1998-2-23
pubmed:abstractText
B-deficient bean (Phaseolus vulgaris L.) nodules examined by light microscopy showed dramatic anatomical changes, mainly in the parenchyma region. Western analysis of total nodule extracts examined by sodium dodecyl sulfate-polyacrylamide gel electrophoresis showed that one 116-kD polypeptide was recognized by antibodies raised against hydroxyproline-rich glycoproteins (HRGPs) from the soybean (Glycine max) seed coat. A protein with a comparable molecular mass of 116 kD was purified from the cell walls of soybean root nodules. The amino acid composition of this protein is similar to the early nodulin (ENOD2) gene. Immunoprecipitation of the soybean ENOD2 in vitro translation product showed that the soybean seed coat anti-HRGP antibodies recognized this early nodulin. Furthermore, we used these antibodies to localize the ENOD2 homolog in bean nodules. Immunocytochemistry revealed that in B-deficient nodules ENOD2 was absent in the walls of the nodule parenchyma. The absence of ENOD2 in B-deficient nodules was corroborated by performing hydroxyproline assays. Northern analysis showed that ENOD2 mRNA is present in B-deficient nodules; therefore, the accumulation of ENOD2 is not affected by B deficiency, but its assembly into the cell wall is. B-deficient nodules fix much less N2 than control nodules, probably because the nodule parenchyma is no longer an effective O2 barrier.
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/9414547-1605832, http://linkedlifedata.com/resource/pubmed/commentcorrection/9414547-16593857, http://linkedlifedata.com/resource/pubmed/commentcorrection/9414547-16659961, http://linkedlifedata.com/resource/pubmed/commentcorrection/9414547-16664769, http://linkedlifedata.com/resource/pubmed/commentcorrection/9414547-16665575, http://linkedlifedata.com/resource/pubmed/commentcorrection/9414547-16667889, http://linkedlifedata.com/resource/pubmed/commentcorrection/9414547-16667932, http://linkedlifedata.com/resource/pubmed/commentcorrection/9414547-1688528, http://linkedlifedata.com/resource/pubmed/commentcorrection/9414547-2297789, http://linkedlifedata.com/resource/pubmed/commentcorrection/9414547-2535534, http://linkedlifedata.com/resource/pubmed/commentcorrection/9414547-3036811, http://linkedlifedata.com/resource/pubmed/commentcorrection/9414547-3422480, http://linkedlifedata.com/resource/pubmed/commentcorrection/9414547-5432063, http://linkedlifedata.com/resource/pubmed/commentcorrection/9414547-7506607, http://linkedlifedata.com/resource/pubmed/commentcorrection/9414547-7556193, http://linkedlifedata.com/resource/pubmed/commentcorrection/9414547-7919991, http://linkedlifedata.com/resource/pubmed/commentcorrection/9414547-8159793, http://linkedlifedata.com/resource/pubmed/commentcorrection/9414547-8439747, http://linkedlifedata.com/resource/pubmed/commentcorrection/9414547-960984
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Dec
pubmed:issn
0032-0889
pubmed:author
pubmed:issnType
Print
pubmed:volume
115
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
1329-40
pubmed:dateRevised
2011-6-29
pubmed:meshHeading
pubmed:year
1997
pubmed:articleTitle
The aberrant cell walls of boron-deficient bean root nodules have no covalently bound hydroxyproline-/proline-rich proteins.
pubmed:affiliation
Departamento de Biología, Facultad de Ciencias, Universidad Autónoma de Madrid, Spain.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't