Linked Life Data

9414321

Source:http://linkedlifedata.com/resource/pubmed/id/9414321

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
12
pubmed:dateCreated
1998-1-20
pubmed:abstractText
The construction of sequence-ready maps of overlapping genomic clones is central to large-scale genome sequencing. We have implemented a method for fluorescent fingerprinting of bacterial clones to assemble contig maps. The method utilizes three spectrally distinct fluorescently tagged dideoxy ATPs to specifically label the HindIII termini in HindIII and Sau3AI restriction digests of clones that are multiplexed prior to electrophoresis and data collection. There is excellent reproducibility of raw data, improved resolution of large fragments, and concordance between the results obtained using this and the equivalent radioactive protocol. This method also allows detection of smaller overlaps between clones when compared to the analysis of restriction digests on nondenaturing agarose gels.
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/9414321-1528894, http://linkedlifedata.com/resource/pubmed/commentcorrection/9414321-1577496, http://linkedlifedata.com/resource/pubmed/commentcorrection/9414321-2737675, http://linkedlifedata.com/resource/pubmed/commentcorrection/9414321-2740339, http://linkedlifedata.com/resource/pubmed/commentcorrection/9414321-2838135, http://linkedlifedata.com/resource/pubmed/commentcorrection/9414321-3463999, http://linkedlifedata.com/resource/pubmed/commentcorrection/9414321-388356, http://linkedlifedata.com/resource/pubmed/commentcorrection/9414321-7601461, http://linkedlifedata.com/resource/pubmed/commentcorrection/9414321-7665188, http://linkedlifedata.com/resource/pubmed/commentcorrection/9414321-7873750, http://linkedlifedata.com/resource/pubmed/commentcorrection/9414321-8136839, http://linkedlifedata.com/resource/pubmed/commentcorrection/9414321-8514151, http://linkedlifedata.com/resource/pubmed/commentcorrection/9414321-8723717, http://linkedlifedata.com/resource/pubmed/commentcorrection/9414321-9367125
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Dec
pubmed:issn
1088-9051
pubmed:author
pubmed:issnType
Print
pubmed:volume
7
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
1162-8
pubmed:dateRevised
2009-11-18
pubmed:meshHeading
pubmed:year
1997
pubmed:articleTitle
Genome mapping by fluorescent fingerprinting.
pubmed:affiliation
The Sanger Centre, Wellcome Trust Genome Campus, Hinxton, Cambridgeshire CB10 1SA, UK. sgg@sanger.ac.uk
pubmed:publicationType
Journal Article