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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
1-2
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pubmed:dateCreated |
1998-1-16
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pubmed:databankReference |
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pubmed:abstractText |
A novel antigen recognized by serum from a scleroderma patient was identified by expression cloning from the HeLa cell cDNA library. The cloned cDNA encoded a 374-amino acid protein with a relative molecular mass of 47,000 and a predicted amino acid sequence 62.7% identical to the hypothetical protein of Caenorhabditis elegans, T16H12.5. The deduced amino acid sequence had a typical POZ domain and an unidentified region conserved during evolution. No zinc finger or RNA recognition motifs were found in this clone. The 2 kbp mRNA encoding the novel clone SPOP (speckle-type POZ protein) was found to be expressed in all human tissues examined. HA-tagged SPOP, transfected and overexpressed in COS7 cells, exhibited a discrete speckled pattern in the nuclei and was co-localized with the splicing factor, snRNP B'/B. Deletion analysis revealed that both the POZ domain and the evolutionarily conserved region at the amino-terminus are required for the nuclear speckled accumulation of SPOP.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Nov
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pubmed:issn |
0014-5793
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
24
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pubmed:volume |
418
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
23-6
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pubmed:dateRevised |
2006-11-20
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pubmed:meshHeading |
pubmed-meshheading:9414087-Amino Acid Sequence,
pubmed-meshheading:9414087-Animals,
pubmed-meshheading:9414087-COS Cells,
pubmed-meshheading:9414087-Caenorhabditis elegans,
pubmed-meshheading:9414087-Cloning, Molecular,
pubmed-meshheading:9414087-Conserved Sequence,
pubmed-meshheading:9414087-DNA, Complementary,
pubmed-meshheading:9414087-Evolution, Molecular,
pubmed-meshheading:9414087-Gene Library,
pubmed-meshheading:9414087-HeLa Cells,
pubmed-meshheading:9414087-Humans,
pubmed-meshheading:9414087-Molecular Sequence Data,
pubmed-meshheading:9414087-Nuclear Proteins,
pubmed-meshheading:9414087-RNA, Messenger,
pubmed-meshheading:9414087-Recombinant Proteins,
pubmed-meshheading:9414087-Repressor Proteins,
pubmed-meshheading:9414087-Sequence Alignment,
pubmed-meshheading:9414087-Sequence Homology, Amino Acid,
pubmed-meshheading:9414087-Sequence Tagged Sites,
pubmed-meshheading:9414087-Transcription, Genetic,
pubmed-meshheading:9414087-Transfection
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pubmed:year |
1997
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pubmed:articleTitle |
Identification of a novel nuclear speckle-type protein, SPOP.
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pubmed:affiliation |
Department of Endocrinology, Medical Research Institute, Tokyo Medical and Dental University, Japan.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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