Linked Life Data

9408998

Source:http://linkedlifedata.com/resource/pubmed/id/9408998

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1-2
pubmed:dateCreated
1998-1-27
pubmed:abstractText
In this report we describe the development of a method for the concurrent reversed-phase high-performance liquid chromatographic separation and photodiode array detection of human plasma retinol, tocopherols and carotenes. For a single sample injection, retinol, retinyl acetate, alpha-tocopherol, gamma-tocopherol, alpha-tocopheryl acetate, all-trans-alpha-carotene and all-trans-beta-carotene, as well as the mono-cis geometrical isomers of beta-carotene were separated and detected. Analytical separations were performed at a subambient temperature (0 degree C) over a Suplex pKb-100 reversed-phase analytical column with an isocratic mobile phase of methanol-methyl tert.-butyl ether-water (80:20:5, v/v/v) at a flow-rate of 0.8 ml/min for 60 min. Standards and samples were reconstituted in ethanol, and typically, 50 microliters was injected for analysis. By HPLC, compounds of interest were clearly resolved and detectable at the picomole level.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Nov
pubmed:issn
0021-9673
pubmed:author
pubmed:issnType
Print
pubmed:day
7
pubmed:volume
787
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
111-8
pubmed:dateRevised
2009-1-15
pubmed:meshHeading
pubmed:year
1997
pubmed:articleTitle
Concurrent liquid chromatographic separation and photodiode array detection of retinol, tocopherols, all-trans-alpha-carotene, all-trans-beta-carotene and the mono-cis isomers of beta-carotene in extracts of human plasma.
pubmed:affiliation
Center for Nutrition Research, James H. Quillen College of Medicine, East Tennessee State University, Johnson City 37614-0410, USA.
pubmed:publicationType
Journal Article