Linked Life Data

9408922

Source:http://linkedlifedata.com/resource/pubmed/id/9408922

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
3
pubmed:dateCreated
1998-2-4
pubmed:abstractText
The effects that haemoglobin-metabolite interactions have on estimates of free magnesium-ion concentration in human erythrocytes, determined by 31P NMR [Gupta, R. K. et al., J. Biol. Chem. 253, 6172-6176 (1978)], were investigated. If the metabolite-haemoglobin association constants of Berger et al. [Eur. J. Biochem. 38, 553-562 (1973)] are used in the analysis then the estimates of intracellular free magnesium-ion concentration made by Gupta et al. (0.25 and 0.67 mM) become 0.43 and 0.60 mM, for oxygenated and deoxygenated cells, respectively. In oxygenated cells, this difference is primarily due to the lower value of KHbMgATP, given by Berger et al. These newly calculated concentrations are in closer agreement with those of Flatman (0.40 mM for oxygenated cells; 0.62 mM for deoxygenated cells) [Flatman, P. W., J. Physiol. 300, 19-30 (1980)] obtained with the 'zero-point titration' method. In addition, the assumptions that the chemical shift separations between the alpha- and beta-phosphorus resonances of ATP and MgATP are unchanged on association with Hb were shown to be false. Under normal intracellular conditions this may lead to errors of 5-10%. Much larger errors would be possible in cases where significant amounts of ATP or MgATP are bound to Hb. These outcomes place doubt on measurements of intracellular free Mg2+ concentration made using 31P NMR if there is no consideration given to the total concentration of 2,3-bisphosphoglycerate (BPG), ATP and Hb in the sample; the same principle would apply to other cell-types.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
May
pubmed:issn
0952-3480
pubmed:author
pubmed:issnType
Print
pubmed:volume
10
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
129-37
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed:year
1997
pubmed:articleTitle
Free magnesium-ion concentration in erythrocytes by 31P NMR: the effect of metabolite-haemoglobin interactions.
pubmed:affiliation
Department of Biochemistry, University of Sydney, NSW, Australia.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't