| pubmed-article:9408264 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:9408264 | lifeskim:mentions | umls-concept:C0007452 | lld:lifeskim |
| pubmed-article:9408264 | lifeskim:mentions | umls-concept:C0018120 | lld:lifeskim |
| pubmed-article:9408264 | lifeskim:mentions | umls-concept:C0221971 | lld:lifeskim |
| pubmed-article:9408264 | lifeskim:mentions | umls-concept:C1571705 | lld:lifeskim |
| pubmed-article:9408264 | lifeskim:mentions | umls-concept:C0023607 | lld:lifeskim |
| pubmed-article:9408264 | lifeskim:mentions | umls-concept:C0033554 | lld:lifeskim |
| pubmed-article:9408264 | lifeskim:mentions | umls-concept:C1171362 | lld:lifeskim |
| pubmed-article:9408264 | lifeskim:mentions | umls-concept:C0017262 | lld:lifeskim |
| pubmed-article:9408264 | lifeskim:mentions | umls-concept:C1515670 | lld:lifeskim |
| pubmed-article:9408264 | lifeskim:mentions | umls-concept:C0560020 | lld:lifeskim |
| pubmed-article:9408264 | lifeskim:mentions | umls-concept:C0205227 | lld:lifeskim |
| pubmed-article:9408264 | pubmed:issue | 6 | lld:pubmed |
| pubmed-article:9408264 | pubmed:dateCreated | 1998-2-11 | lld:pubmed |
| pubmed-article:9408264 | pubmed:abstractText | To determine whether prostaglandin G/H synthase-2 (PGHS-2) induction occurs under physiological conditions, heifers were ovariectomized and preovulatory follicles were isolated 0 (n = 4), 18 (n = 4), and 24 h (n = 4) after the onset of estrus. The time of the LH surge was determined in blood samples collected every 4 h pre- and postestrus. Preparations of follicle wall and isolated theca interna and granulosa cells were analyzed for PGHS-2 protein and mRNA by immunoblot and Northern blot, respectively. Immunohistochemistry was used to document the in situ localization of PGHS-2 protein, and follicular fluid concentrations of prostaglandin (PG) E2 and PGF2alpha were determined to monitor changes in PG synthetic activities. Results showed that PGHS-2 mRNA (4.0 kilobases) and protein (74000 Mr) were absent in preovulatory follicles isolated at the onset of estrus, low in follicles obtained 18 h after estrus (16.0 +/- 1.2 h post-LH surge), and markedly induced 24 h postestrus (20 +/- 0 h post-LH surge). Immunoblot and immunohistochemical analyses revealed that PGHS-2 protein was selectively induced in granulosa cells. Follicular fluid concentrations of PGE2 and PGF2alpha increased significantly (p < 0.01) between 0 and 24 h after estrus (from 2.8 +/- 0.2 to 87.9 +/- 30.9 ng/ml for PGE2; from 0.05 +/- 0.02 to 68.9 +/- 23.6 ng/ml for PGF2alpha). Collectively, these results clearly demonstrate that the induction of PGHS-2 in bovine preovulatory follicles is a physiological event that occurs after the endogenous LH surge. | lld:pubmed |
| pubmed-article:9408264 | pubmed:language | eng | lld:pubmed |
| pubmed-article:9408264 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:9408264 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:9408264 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:9408264 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:9408264 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:9408264 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:9408264 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:9408264 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:9408264 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:9408264 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:9408264 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:9408264 | pubmed:month | Dec | lld:pubmed |
| pubmed-article:9408264 | pubmed:issn | 0006-3363 | lld:pubmed |
| pubmed-article:9408264 | pubmed:author | pubmed-author:LiuJJ | lld:pubmed |
| pubmed-article:9408264 | pubmed:author | pubmed-author:SiroisJJ | lld:pubmed |
| pubmed-article:9408264 | pubmed:author | pubmed-author:DoréMM | lld:pubmed |
| pubmed-article:9408264 | pubmed:author | pubmed-author:CarrièreP DPD | lld:pubmed |
| pubmed-article:9408264 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:9408264 | pubmed:volume | 57 | lld:pubmed |
| pubmed-article:9408264 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:9408264 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:9408264 | pubmed:pagination | 1524-31 | lld:pubmed |
| pubmed-article:9408264 | pubmed:dateRevised | 2006-11-15 | lld:pubmed |
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| pubmed-article:9408264 | pubmed:year | 1997 | lld:pubmed |
| pubmed-article:9408264 | pubmed:articleTitle | Prostaglandin G/H synthase-2 is expressed in bovine preovulatory follicles after the endogenous surge of luteinizing hormone. | lld:pubmed |
| pubmed-article:9408264 | pubmed:affiliation | Centre de recherche en reproduction animale, Faculté de médecine vétérinaire, Université de Montréal, Saint-Hyacinthe, Québec, Canada. | lld:pubmed |
| pubmed-article:9408264 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:9408264 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:9408264 | lld:pubmed |
