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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
6
|
| pubmed:dateCreated |
1998-2-11
|
| pubmed:abstractText |
To determine whether prostaglandin G/H synthase-2 (PGHS-2) induction occurs under physiological conditions, heifers were ovariectomized and preovulatory follicles were isolated 0 (n = 4), 18 (n = 4), and 24 h (n = 4) after the onset of estrus. The time of the LH surge was determined in blood samples collected every 4 h pre- and postestrus. Preparations of follicle wall and isolated theca interna and granulosa cells were analyzed for PGHS-2 protein and mRNA by immunoblot and Northern blot, respectively. Immunohistochemistry was used to document the in situ localization of PGHS-2 protein, and follicular fluid concentrations of prostaglandin (PG) E2 and PGF2alpha were determined to monitor changes in PG synthetic activities. Results showed that PGHS-2 mRNA (4.0 kilobases) and protein (74000 Mr) were absent in preovulatory follicles isolated at the onset of estrus, low in follicles obtained 18 h after estrus (16.0 +/- 1.2 h post-LH surge), and markedly induced 24 h postestrus (20 +/- 0 h post-LH surge). Immunoblot and immunohistochemical analyses revealed that PGHS-2 protein was selectively induced in granulosa cells. Follicular fluid concentrations of PGE2 and PGF2alpha increased significantly (p < 0.01) between 0 and 24 h after estrus (from 2.8 +/- 0.2 to 87.9 +/- 30.9 ng/ml for PGE2; from 0.05 +/- 0.02 to 68.9 +/- 23.6 ng/ml for PGF2alpha). Collectively, these results clearly demonstrate that the induction of PGHS-2 in bovine preovulatory follicles is a physiological event that occurs after the endogenous LH surge.
|
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Dinoprost,
http://linkedlifedata.com/resource/pubmed/chemical/Dinoprostone,
http://linkedlifedata.com/resource/pubmed/chemical/Estradiol,
http://linkedlifedata.com/resource/pubmed/chemical/Isoenzymes,
http://linkedlifedata.com/resource/pubmed/chemical/Luteinizing Hormone,
http://linkedlifedata.com/resource/pubmed/chemical/Progesterone,
http://linkedlifedata.com/resource/pubmed/chemical/Prostaglandin-Endoperoxide Synthases,
http://linkedlifedata.com/resource/pubmed/chemical/RNA, Messenger
|
| pubmed:status |
MEDLINE
|
| pubmed:month |
Dec
|
| pubmed:issn |
0006-3363
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:volume |
57
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
1524-31
|
| pubmed:dateRevised |
2006-11-15
|
| pubmed:meshHeading |
pubmed-meshheading:9408264-Animals,
pubmed-meshheading:9408264-Blotting, Northern,
pubmed-meshheading:9408264-Cattle,
pubmed-meshheading:9408264-Dinoprost,
pubmed-meshheading:9408264-Dinoprostone,
pubmed-meshheading:9408264-Estradiol,
pubmed-meshheading:9408264-Estrus,
pubmed-meshheading:9408264-Female,
pubmed-meshheading:9408264-Follicular Fluid,
pubmed-meshheading:9408264-Gene Expression,
pubmed-meshheading:9408264-Immunoblotting,
pubmed-meshheading:9408264-Immunohistochemistry,
pubmed-meshheading:9408264-Isoenzymes,
pubmed-meshheading:9408264-Luteinizing Hormone,
pubmed-meshheading:9408264-Ovarian Follicle,
pubmed-meshheading:9408264-Ovulation,
pubmed-meshheading:9408264-Progesterone,
pubmed-meshheading:9408264-Prostaglandin-Endoperoxide Synthases,
pubmed-meshheading:9408264-RNA, Messenger
|
| pubmed:year |
1997
|
| pubmed:articleTitle |
Prostaglandin G/H synthase-2 is expressed in bovine preovulatory follicles after the endogenous surge of luteinizing hormone.
|
| pubmed:affiliation |
Centre de recherche en reproduction animale, Faculté de médecine vétérinaire, Université de Montréal, Saint-Hyacinthe, Québec, Canada.
|
| pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
|