Linked Life Data

9407094

Source:http://linkedlifedata.com/resource/pubmed/id/9407094

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
52
pubmed:dateCreated
1998-1-23
pubmed:abstractText
The cDNA encoding hmGluR6, appended with a 15-amino acid antibody epitope (1D4), was transiently transfected in COS-7 cells. The receptor was purified from COS cell membranes using an antibody affinity column. The purified receptor was then reconstituted into lipid vesicles, and its ability to activate either transducin, the rod photoreceptor-specific GTP-binding protein, or the alpha subunit of Go was assayed in vitro using a guanosine 5'-3-O-(thio)triphosphate binding assay. Activation of both transducin and Go was observed. The rate of Go activation was 18-fold greater than the rate of transducin activation. This indicates that the coupling of mGluR6 to Go is more efficient and suggests that Go may be involved in coupling to mGluR6 in ON-bipolar cells.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Dec
pubmed:issn
0021-9258
pubmed:author
pubmed:issnType
Print
pubmed:day
26
pubmed:volume
272
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
33100-4
pubmed:dateRevised
2009-11-19
pubmed:meshHeading
pubmed-meshheading:9407094-Amino Acid Sequence, pubmed-meshheading:9407094-Aminobutyric Acids, pubmed-meshheading:9407094-Animals, pubmed-meshheading:9407094-Anticonvulsants, pubmed-meshheading:9407094-COS Cells, pubmed-meshheading:9407094-Cattle, pubmed-meshheading:9407094-Excitatory Amino Acid Agonists, pubmed-meshheading:9407094-GTP-Binding Protein alpha Subunits, Gi-Go, pubmed-meshheading:9407094-GTP-Binding Proteins, pubmed-meshheading:9407094-Guanosine 5'-O-(3-Thiotriphosphate), pubmed-meshheading:9407094-Guanosine Triphosphate, pubmed-meshheading:9407094-Humans, pubmed-meshheading:9407094-Molecular Sequence Data, pubmed-meshheading:9407094-Rats, pubmed-meshheading:9407094-Receptors, Metabotropic Glutamate, pubmed-meshheading:9407094-Transducin, pubmed-meshheading:9407094-Transfection
pubmed:year
1997
pubmed:articleTitle
Functional coupling of a human retinal metabotropic glutamate receptor (hmGluR6) to bovine rod transducin and rat Go in an in vitro reconstitution system.
pubmed:affiliation
Department of Biological Sciences, University of Maryland Baltimore County, Baltimore, Maryland 21250, USA.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S.