Linked Life Data

9407004

Source:http://linkedlifedata.com/resource/pubmed/id/9407004

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
11
pubmed:dateCreated
1998-1-6
pubmed:abstractText
Conserved regions 1 and 2 as well as the amino terminus of E1A are required for the transforming activity of the E1A oncoprotein. We show here that the amino terminus of 243R E1A has transactivation activity when brought to a promoter in yeast. Recruitment to a specific promoter is essential. Mutagenesis studies correlated the transactivation function with the extreme amino terminus and the conserved region 1 of E1A. Cotransfection assays in rodent cells confirmed that two overlapping but distinguishable domains, amino acids 1-65 and 37-80, can transactivate independently when targeted to a promoter. We also observed that when recruited to the proliferating cell nuclear antigen (PCNA) promoter, the amino-terminal region was sufficient to transactivate the PCNA promoter. On the other hand, deletion of the amino terminus of E1A resulted in failure to induce PCNA expression. Fusion of VP16 with the amino-terminal-deleted E1A mutant was able to restore the ability to induce the PCNA promoter. We further show that the amino-terminal region also is required for 243R E1A to repress the transactivation mediated by a universal transactivator DBD.VP16 and DBD.E1A. This repression could be specifically relieved by overexpression of TBP but not TFIIB. In addition, we show that the amino terminus of E1A is involved in in vitro interaction with the TATA binding protein (TBP). Thus the amino-terminal transforming region of E1A may regulate cellular gene expression in species that are distant in evolution via a common mechanism, functionally targeting TBP.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Nov
pubmed:issn
1044-5498
pubmed:author
pubmed:issnType
Print
pubmed:volume
16
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
1321-33
pubmed:dateRevised
2008-11-21
pubmed:meshHeading
pubmed-meshheading:9407004-3T3 Cells, pubmed-meshheading:9407004-Adenovirus E1A Proteins, pubmed-meshheading:9407004-Animals, pubmed-meshheading:9407004-Binding Sites, pubmed-meshheading:9407004-DNA-Binding Proteins, pubmed-meshheading:9407004-Gene Expression Regulation, Fungal, pubmed-meshheading:9407004-Herpes Simplex Virus Protein Vmw65, pubmed-meshheading:9407004-Mice, pubmed-meshheading:9407004-Mice, Inbred BALB C, pubmed-meshheading:9407004-Proliferating Cell Nuclear Antigen, pubmed-meshheading:9407004-Promoter Regions, Genetic, pubmed-meshheading:9407004-Saccharomyces cerevisiae, pubmed-meshheading:9407004-TATA-Box Binding Protein, pubmed-meshheading:9407004-Transcription, Genetic, pubmed-meshheading:9407004-Transcription Factors, pubmed-meshheading:9407004-Transcriptional Activation
pubmed:year
1997
pubmed:articleTitle
Transforming region of 243R E1A contains two overlapping but distinct transactivation domains.
pubmed:affiliation
Department of Pathology, Anatomy & Cell Biology, Sbarro Institute for Cancer Research and Molecular Medicine, Jefferson Medical College, Philadelphia, PA 19107, USA.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S.