Linked Life Data

9406867

Source:http://linkedlifedata.com/resource/pubmed/id/9406867

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
1998-2-13
pubmed:databankReference
pubmed:abstractText
The rhythmic daytime inhibition of phagocytosis of shed photoreceptor outer segments (OS) by the retinal pigment epithelium (RPE) is related to increased cAMP in RPE cells. Dopamine (DA), the light-adaptive signal of the retinal oscillator can activate adenylyl cyclase through its D1-like receptors. It reduces OS phagocytosis by cultured bovine RPE, but a DA receptor was not demonstrated. Using primers selected from alignment of D1-like receptor genes already cloned, we have amplified by PCR two sequences in bovine genomic DNA. Phylogenetic analysis demonstrated that they correspond to D1a and D1b receptors. These receptors were then searched for using the reverse transcription-polymerase chain reaction (RT-PCR) in cultured bovine RPE. Only the D1b receptor subtype was demonstrated. It could mediate the DA-induced inhibition of phagocytosis.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Nov
pubmed:issn
0304-3940
pubmed:author
pubmed:issnType
Print
pubmed:day
14
pubmed:volume
237
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
9-12
pubmed:dateRevised
2005-11-17
pubmed:meshHeading
pubmed:year
1997
pubmed:articleTitle
Molecular identification of a dopamine D1b receptor in bovine retinal pigment epithelium.
pubmed:affiliation
Laboratoire de NeuroCytologie Oculaire, INSERM U-86, Paris, France.
pubmed:publicationType
Journal Article