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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
26
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pubmed:dateCreated |
1998-2-2
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pubmed:abstractText |
The efficient introduction of somatic mutations in a given gene, at a given time, in a specific cell type will facilitate studies of gene function and the generation of animal models for human diseases. We have shown previously that conditional recombination-excision between two loxP sites can be achieved in mice by using the Cre recombinase fused to a mutated ligand binding domain of the human estrogen receptor (Cre-ERT), which binds tamoxifen but not estrogens. DNA excision was induced in a number of tissues after administration of tamoxifen to transgenic mice expressing Cre-ERT under the control of the cytomegalovirus promoter. However, the efficiency of excision varied between tissues, and the highest level ( approximately 40%) was obtained in the skin. To determine the efficiency of excision mediated by Cre-ERT in a given cell type, we have now crossed Cre-ERT-expressing mice with reporter mice in which expression of Escherichia coli beta-galactosidase can be induced through Cre-mediated recombination. The efficiency and kinetics of this recombination were analyzed at the cellular level in the epidermis of 6- to 8-week-old double transgenic mice. We show that site-specific excision occurred within a few days of tamoxifen treatment in essentially all epidermis cells expressing Cre-ERT. These results indicate that cell-specific expression of Cre-ERT in transgenic mice can be used for efficient tamoxifen-dependent, Cre-mediated recombination at loci containing loxP sites to generate site-specific somatic mutations in a spatio-temporally controlled manner.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/9405652-2164640,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9405652-2269655,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9405652-2660363,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9405652-2783482,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9405652-3287623,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9405652-7624356,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9405652-7660125,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9405652-7816809,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9405652-8016642,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9405652-8620528,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9405652-8698848,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9405652-8855277,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9405652-8962131,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9405652-8980237,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9405652-9108159,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9405652-9171115
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Dec
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pubmed:issn |
0027-8424
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
23
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pubmed:volume |
94
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
14559-63
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:9405652-Animals,
pubmed-meshheading:9405652-Epidermis,
pubmed-meshheading:9405652-Gene Targeting,
pubmed-meshheading:9405652-Humans,
pubmed-meshheading:9405652-Integrases,
pubmed-meshheading:9405652-Mice,
pubmed-meshheading:9405652-Mice, Transgenic,
pubmed-meshheading:9405652-Mutagenesis, Site-Directed,
pubmed-meshheading:9405652-Receptors, Estrogen,
pubmed-meshheading:9405652-Recombinant Fusion Proteins,
pubmed-meshheading:9405652-Recombination, Genetic,
pubmed-meshheading:9405652-Tamoxifen,
pubmed-meshheading:9405652-Viral Proteins
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pubmed:year |
1997
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pubmed:articleTitle |
Spatio-temporally controlled site-specific somatic mutagenesis in the mouse.
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pubmed:affiliation |
Centre National de la Recherche Scientifique, Institut National de la Santé et de la Recherche Médicale, Université Louis Pasteur, Collège de France, Strasbourg, France.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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