Linked Life Data

9405444

Source:http://linkedlifedata.com/resource/pubmed/id/9405444

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
51
pubmed:dateCreated
1998-1-22
pubmed:databankReference
pubmed:abstractText
To characterize at the molecular level the pancreatic emergency program set up by the pancreatic cells in response to pancreatitis, we have developed a strategy in which the phenotype of the pancreatitis affected pancreas is established by characterization of a large number of its transcripts. Herein, we describe the cloning, sequence, and expression of a new gene, named p8, which is strongly activated in pancreatic acinar cells during the acute phase of pancreatitis, in developing pancreas and during pancreatic regeneration. In acinar cells, p8 mRNA is expressed rapidly and specifically in response to cellular pancreatitis-induced injury; its induction occurred almost similarly in edematous and necrohemorrhagic pancreatitis, indicating that p8 mRNA is maximally activated even in response to a mild pancreatic injury. Furthermore, in vitro studies suggest that p8 mRNA is induced in pancreatic and non-pancreatic cells in response to some apoptotic stimuli. p8 acts as a promoter of cellular growth factor when its cDNA is transfected into COS-7 and AR4-2J cells. Although we failed to identify p8-related sequences, analysis of its primary and secondary structure suggests that p8 is a basic helix-turn-helix-containing gene with slight homology to several homeotic genes and sufficient signal to be targeted to the nucleus. We therefore propose p8 as a putative transcriptional factor which can regulate pancreatic growth.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Dec
pubmed:issn
0021-9258
pubmed:author
pubmed:issnType
Print
pubmed:day
19
pubmed:volume
272
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
32360-9
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed-meshheading:9405444-Amino Acid Sequence, pubmed-meshheading:9405444-Animals, pubmed-meshheading:9405444-Base Sequence, pubmed-meshheading:9405444-Basic Helix-Loop-Helix Transcription Factors, pubmed-meshheading:9405444-Cell Division, pubmed-meshheading:9405444-Cell Line, pubmed-meshheading:9405444-Cloning, Molecular, pubmed-meshheading:9405444-DNA, Complementary, pubmed-meshheading:9405444-DNA-Binding Proteins, pubmed-meshheading:9405444-Gene Expression Regulation, pubmed-meshheading:9405444-Growth Substances, pubmed-meshheading:9405444-In Situ Hybridization, Fluorescence, pubmed-meshheading:9405444-Molecular Sequence Data, pubmed-meshheading:9405444-Neoplasm Proteins, pubmed-meshheading:9405444-Pancreas, pubmed-meshheading:9405444-Pancreatitis, pubmed-meshheading:9405444-RNA, Messenger, pubmed-meshheading:9405444-Rats
pubmed:year
1997
pubmed:articleTitle
Cloning and expression of the rat p8 cDNA, a new gene activated in pancreas during the acute phase of pancreatitis, pancreatic development, and regeneration, and which promotes cellular growth.
pubmed:affiliation
U.315 INSERM, 46 boulevard de la Gaye, F-13009 Marseille, France.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't