Linked Life Data

9405178

Source:http://linkedlifedata.com/resource/pubmed/id/9405178

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
11
pubmed:dateCreated
1998-2-23
pubmed:abstractText
Dihydropyridine-sensitive voltage-gated (l-type) Ca2+ channels play an essential role in cardiac and smooth muscle excitation-contraction coupling. Transcripts for the two pore-forming subunits of l-type Ca2+ channels, alpha 1C and alpha 1D have been detected in heart and lung; however, distribution, structure and regulated expression of these channel subunit mRNAs have not been examined in detail. Here we use RNase protection and RT-PCR assays to identify cardiac-specific features of expression of the two channel mRNAs. First, expression of alpha 1D mRNA is found in lung, aorta and atrium, but is not detected in ventricle. Limited expression of alpha 1D mRNA is also seen in enriched preparations of cardiac myocytes: it is significant in atrial myocytes, but not in ventricular myocytes. Second, RT-PCR analyses indicate that atrial alpha 1D channels exclusively contains the 15-amino acid insertion between third and fourth segments in repeat IV. Finally, expression of alpha 1C mRNA, but not alpha 1D mRNA, is up-regulated by glucocorticoids in atrium and ventricle: adrenalectomy and subsequent injection of the glucocorticoid agonist dexamethasone decreased and increased the channel message, respectively. Dexamethasone also significantly increased the number of dihydropyridine-binding sites in ventricle. In contrast, alpha 1C mRNA levels were glucocorticoid-insensitive in lung and aorta. Thus, basal and glucocorticoid-induced expression, and splicing of the two l-type Ca2+ channel alpha 1 subunit transcripts are tissue specifically controlled in atria and ventricles of rat heart.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Nov
pubmed:issn
0022-2828
pubmed:author
pubmed:copyrightInfo
Copyright 1997 Academic Press Limited.
pubmed:issnType
Print
pubmed:volume
29
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
3035-42
pubmed:dateRevised
2007-11-14
pubmed:meshHeading
pubmed:year
1997
pubmed:articleTitle
Distribution, splicing and glucocorticoid-induced expression of cardiac alpha 1C and alpha 1D voltage-gated Ca2+ channel mRNAs.
pubmed:affiliation
Department of Pharmacology, University of Pittsburgh, PA, 15261, USA.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S.