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rdf:type |
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lifeskim:mentions |
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pubmed:dateCreated |
1998-2-3
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pubmed:abstractText |
1. Whole-cell current-clamp recordings demonstrate that leptin (0.3-10 nm) hyperpolarizes CRI-G1 insulin-secreting cells. This effect is slow on onset and is not reversed on washout of the leptin. 2. Voltage-clamp recordings indicate that leptin activates a potassium conductance in the presence of intracellular ATP (5 mm), but has not effect in its absence. Following activation of ATP-sensitive K+ (KATP) current by diazoxide (0.2 mm), addition of leptin did not alter cell membrane potential or potassium current further. 3. The leptin-induced hyperpolarization and increased potassium conductance are completely inhibited by the application of the sulphonylureas tolbutamide (100 microM) and glibenclamide (0.5 microM). 4. Cell-attached and inside-out single-channel recordings indicate that leptin activates tolbutamide-sensitive KATP channels in CRI-G1 insulin-secreting cells.
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pubmed:grant |
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/9401961-1422577,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9401961-1482696,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9401961-2119205,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9401961-2431383,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9401961-2484976,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9401961-3011941,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9401961-3146398,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9401961-7566151,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9401961-7592639,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9401961-7624776,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9401961-7624777,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9401961-7624778,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9401961-7789654,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9401961-7984236,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9401961-8548812,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9401961-8568673,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9401961-8607834,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9401961-8621022,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9401961-8628397,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9401961-8643697,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9401961-8702421,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9401961-8823291,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9401961-8866547,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9401961-8971096,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9401961-9000710
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Nov
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pubmed:issn |
0022-3751
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
1
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pubmed:volume |
504 ( Pt 3)
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
527-35
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pubmed:dateRevised |
2011-11-17
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pubmed:meshHeading |
pubmed-meshheading:9401961-Adenosine Triphosphate,
pubmed-meshheading:9401961-Animals,
pubmed-meshheading:9401961-Biotransformation,
pubmed-meshheading:9401961-Cell Line,
pubmed-meshheading:9401961-Electrophysiology,
pubmed-meshheading:9401961-Hypoglycemic Agents,
pubmed-meshheading:9401961-Insulin,
pubmed-meshheading:9401961-Ion Channel Gating,
pubmed-meshheading:9401961-Leptin,
pubmed-meshheading:9401961-Membrane Potentials,
pubmed-meshheading:9401961-Patch-Clamp Techniques,
pubmed-meshheading:9401961-Potassium Channels,
pubmed-meshheading:9401961-Proteins,
pubmed-meshheading:9401961-Rats,
pubmed-meshheading:9401961-Tolbutamide
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pubmed:year |
1997
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pubmed:articleTitle |
Leptin activates ATP-sensitive potassium channels in the rat insulin-secreting cell line, CRI-G1.
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pubmed:affiliation |
Department of Biomedical Sciences, University of Aberdeen, UK.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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