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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
3
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pubmed:dateCreated |
1997-12-30
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pubmed:abstractText |
One of the characteristic features of celiac disease is an increase in anti-gliadin antibodies (Abs). Recently we found that some of the monoclonal Abs to gliadin cross-react with molecules on rat enterocytes. One of these cross-reacting molecules was identified as rat calreticulin. This study shows that the levels of serum IgA Abs to gliadin, rat, and human enterocytes; purified enterocyte antigens; and calreticulin in sera from patients with active disease were significantly higher than in patients on a gluten-free diet and healthy controls (P < 0.001). Anti-gliadin Abs were isolated by affinity chromatography from the sera of six active celiac patients. The reactivity of these anti-gliadin Abs was demonstrated to be significantly higher (P < 0.05) with human enterocytes and human calreticulin than with other antigens tested. Furthermore, using isolated patients' anti-gliadin Abs bound to Sepharose 2B, two main proteins of molecular mass 62 and 66 kDa were purified from a lysate of human enterocytes. The 62-kDa enterocyte antigen was identified as human calreticulin. These findings suggest that anti-gliadin Abs may play a pathogenic role in celiac disease by cross-reacting with enterocytes. Calreticulin in enterocytes may be one of the putative targets for autoimmune reactions.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Dec
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pubmed:issn |
0090-1229
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
85
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
289-96
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:9400629-Adolescent,
pubmed-meshheading:9400629-Adult,
pubmed-meshheading:9400629-Aged,
pubmed-meshheading:9400629-Amino Acid Sequence,
pubmed-meshheading:9400629-Animals,
pubmed-meshheading:9400629-Antibodies,
pubmed-meshheading:9400629-Antigens,
pubmed-meshheading:9400629-Calcium-Binding Proteins,
pubmed-meshheading:9400629-Calreticulin,
pubmed-meshheading:9400629-Celiac Disease,
pubmed-meshheading:9400629-Child,
pubmed-meshheading:9400629-Child, Preschool,
pubmed-meshheading:9400629-Cross Reactions,
pubmed-meshheading:9400629-Enzyme-Linked Immunosorbent Assay,
pubmed-meshheading:9400629-Female,
pubmed-meshheading:9400629-Gliadin,
pubmed-meshheading:9400629-Humans,
pubmed-meshheading:9400629-Immunoglobulin A,
pubmed-meshheading:9400629-Immunohistochemistry,
pubmed-meshheading:9400629-Infant,
pubmed-meshheading:9400629-Male,
pubmed-meshheading:9400629-Middle Aged,
pubmed-meshheading:9400629-Molecular Sequence Data,
pubmed-meshheading:9400629-Rats,
pubmed-meshheading:9400629-Rats, Wistar,
pubmed-meshheading:9400629-Ribonucleoproteins
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pubmed:year |
1997
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pubmed:articleTitle |
Anti-gliadin antibodies in patients with celiac disease cross-react with enterocytes and human calreticulin.
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pubmed:affiliation |
Division of Immunology and Gnotobiology, Czech Academy of Sciences, Prague, Czech Republic.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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