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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
2
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pubmed:dateCreated |
1998-1-8
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pubmed:abstractText |
Despite differences in T cell responses induced by interleukin (IL)-2 and IL-7, both cytokines modulate T cell functions by activation of signal transducers and activators of transcription (STAT) proteins. We examined the contribution of the two isoforms of STAT5, STAT5A and STAT5B, to IL-2- and IL-7-induced activation of human peripheral blood T lymphoblasts. Both cytokines induced assembly of STAT5A and STAT5B containing complexes capable of binding to the interferon-gamma activation sequence (GAS), and these complexes rapidly translocated (within 1 min) into the nucleus of IL-2- or IL-7-treated cells. The kinetics of this translocation were delayed in IL-7-treated as compared to IL-2-treated cells. IL-2 and IL-7 were equivalent in their ability to induce tyrosine phosphorylation of STAT5A and STAT5B and to facilitate binding of these STATs to an immobilized GAS element. Both IL-2 and IL-7 induced substantial amounts of STAT5A/STAT5B heterodimerization. Moreover, we observed constitutive association of STAT3 with each STAT5 isomer. These data suggest that IL-2 and IL-7 induce assembly of STAT heterodimers in a similar manner and that subsequent cellular responses may be driven by induction of similar sets of genes.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/DNA,
http://linkedlifedata.com/resource/pubmed/chemical/DNA-Binding Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Interleukin-2,
http://linkedlifedata.com/resource/pubmed/chemical/Interleukin-7,
http://linkedlifedata.com/resource/pubmed/chemical/Isoenzymes,
http://linkedlifedata.com/resource/pubmed/chemical/Milk Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Recombinant Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/STAT5 Transcription Factor,
http://linkedlifedata.com/resource/pubmed/chemical/STAT5A protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/STAT5B protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/Stat5a protein, mouse,
http://linkedlifedata.com/resource/pubmed/chemical/Stat5b protein, mouse,
http://linkedlifedata.com/resource/pubmed/chemical/Trans-Activators,
http://linkedlifedata.com/resource/pubmed/chemical/Tumor Suppressor Proteins
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pubmed:status |
MEDLINE
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pubmed:month |
Nov
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pubmed:issn |
0008-8749
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
1
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pubmed:volume |
181
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
172-81
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pubmed:dateRevised |
2008-11-21
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pubmed:meshHeading |
pubmed-meshheading:9398404-Animals,
pubmed-meshheading:9398404-Cell Nucleus,
pubmed-meshheading:9398404-Cells, Cultured,
pubmed-meshheading:9398404-DNA,
pubmed-meshheading:9398404-DNA-Binding Proteins,
pubmed-meshheading:9398404-Dimerization,
pubmed-meshheading:9398404-Electrophoresis, Polyacrylamide Gel,
pubmed-meshheading:9398404-Gene Expression Regulation,
pubmed-meshheading:9398404-Humans,
pubmed-meshheading:9398404-Interleukin-2,
pubmed-meshheading:9398404-Interleukin-7,
pubmed-meshheading:9398404-Isoenzymes,
pubmed-meshheading:9398404-Mice,
pubmed-meshheading:9398404-Milk Proteins,
pubmed-meshheading:9398404-Promoter Regions, Genetic,
pubmed-meshheading:9398404-Protein Multimerization,
pubmed-meshheading:9398404-Recombinant Proteins,
pubmed-meshheading:9398404-Regulatory Sequences, Nucleic Acid,
pubmed-meshheading:9398404-STAT5 Transcription Factor,
pubmed-meshheading:9398404-Signal Transduction,
pubmed-meshheading:9398404-T-Lymphocytes,
pubmed-meshheading:9398404-Trans-Activators,
pubmed-meshheading:9398404-Tumor Suppressor Proteins
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pubmed:year |
1997
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pubmed:articleTitle |
IL-2 and IL-7 induce heterodimerization of STAT5 isoforms in human peripheral blood T lymphoblasts.
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pubmed:affiliation |
Division of Cytokine Biology, Food and Drug Administration, Bethesda, Maryland 20892-4555, USA.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, U.S. Gov't, Non-P.H.S.
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