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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
1
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pubmed:dateCreated |
1998-1-27
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pubmed:abstractText |
A Gill titration calorimeter is evaluated as an instrument to determine in one experiment the equilibrium constant and the enthalpy change of a biochemical reaction. The dimensionless parameter kc (the product of the association equilibrium constant and the concentration of the reagent to be titrated; Wiseman et al., Anal. Biochem. 179, 131-137, 1989) is used to analyze the instrument performance. The analysis of simulated titration data corresponding to a simple model case shows that association equilibrium constants in the 10(2)-10(7) M-1 range may be determined when the kc parameter is between 1 and 1000. In addition we use a Monte Carlo approach to estimate the precision in the thermodynamic parameters of the reaction under study. The relative precision in the calculated constants ranges from 3 to 80% depending on the macromolecule concentration and kc value in the experiment. These results were checked with the study of the reactions of beta-trypsin with its inhibitor and ribonuclease A with cytidine 2'-monophosphate and cytidine 3'-monophosphate.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Dec
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pubmed:issn |
0003-2697
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pubmed:author |
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pubmed:copyrightInfo |
Copyright 1997 Academic Press.
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pubmed:issnType |
Print
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pubmed:day |
1
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pubmed:volume |
254
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
96-108
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pubmed:dateRevised |
2008-11-21
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pubmed:meshHeading |
pubmed-meshheading:9398351-Binding Sites,
pubmed-meshheading:9398351-Calorimetry,
pubmed-meshheading:9398351-Cytidine Monophosphate,
pubmed-meshheading:9398351-Enzyme Inhibitors,
pubmed-meshheading:9398351-Enzymes,
pubmed-meshheading:9398351-Kinetics,
pubmed-meshheading:9398351-Monte Carlo Method,
pubmed-meshheading:9398351-Plant Proteins,
pubmed-meshheading:9398351-Protein Binding,
pubmed-meshheading:9398351-Ribonuclease, Pancreatic,
pubmed-meshheading:9398351-Thermodynamics,
pubmed-meshheading:9398351-Titrimetry,
pubmed-meshheading:9398351-Trypsin,
pubmed-meshheading:9398351-Trypsin Inhibitors,
pubmed-meshheading:9398351-alpha-Amylases
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pubmed:year |
1997
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pubmed:articleTitle |
Measurement of biochemical affinities with a Gill titration calorimeter.
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pubmed:affiliation |
Facultad de Ciencias, Universidad de Granada, Granada, 18071, Spain.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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