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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
6
|
| pubmed:dateCreated |
1998-1-8
|
| pubmed:abstractText |
C-Myb is a sequence-specific DNA binding protein that regulates the expression of genes involved in cell proliferation and differentiation. The present study was designed to elucidate the role of c-Myb in the pathogenesis of chronic liver disease by an immunohistochemical approach. In normal (control) livers, few or no hepatic cells were positive for c-Myb. In livers from patients with chronic viral hepatitis, positive staining for c-Myb was found not only in spindle-shaped mesenchymal cells in expanded portal areas, but also in perisinusoidal cells (PSCs) within liver lobules. In cirrhotic livers, a few PSCs within lobules were positive for c-Myb, while no staining was seen in fibrous septa. Immunoelectron microscopy revealed that c-Myb-positive PSCs displayed morphological features of hepatic stellate cells. Other sinusoidal lining cells including Kupffer cells and sinusoidal endothelial cells, as well as hepatocytes, were all negative for c-Myb. Dual c-Myb/alpha-smooth muscle actin (alphaSMA) staining revealed that more than 97% of c-Myb-positive cells were alphaSMA-positive. Moreover, dual c-Myb/proliferating cell nuclear antigen (PCNA) staining showed that approximately 70% of c-Myb-positive cells also expressed PCNA. The labeling index (LI) (number of c-Myb-positive cells/0.1 mm2) significantly correlated with serum transaminase concentrations and increased in parallel with the disease activity. However, the LI showed no correlation with the degree of fibrosis. These results suggest that c-Myb may be involved in stellate cell activation and proliferation in chronically diseased human livers, and that the level of c-Myb expression is associated with the activity of chronic hepatitis.
|
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Actins,
http://linkedlifedata.com/resource/pubmed/chemical/Antibodies, Monoclonal,
http://linkedlifedata.com/resource/pubmed/chemical/Proto-Oncogene Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Proto-Oncogene Proteins c-myb,
http://linkedlifedata.com/resource/pubmed/chemical/Trans-Activators
|
| pubmed:status |
MEDLINE
|
| pubmed:month |
Dec
|
| pubmed:issn |
0270-9139
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:volume |
26
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
1506-12
|
| pubmed:dateRevised |
2006-11-15
|
| pubmed:meshHeading |
pubmed-meshheading:9397991-Actins,
pubmed-meshheading:9397991-Adult,
pubmed-meshheading:9397991-Antibodies, Monoclonal,
pubmed-meshheading:9397991-Antibody Specificity,
pubmed-meshheading:9397991-Biopsy,
pubmed-meshheading:9397991-Blotting, Western,
pubmed-meshheading:9397991-Chronic Disease,
pubmed-meshheading:9397991-Female,
pubmed-meshheading:9397991-Hepatitis, Viral, Human,
pubmed-meshheading:9397991-Humans,
pubmed-meshheading:9397991-Immunohistochemistry,
pubmed-meshheading:9397991-Liver,
pubmed-meshheading:9397991-Liver Cirrhosis,
pubmed-meshheading:9397991-Liver Diseases,
pubmed-meshheading:9397991-Liver Function Tests,
pubmed-meshheading:9397991-Male,
pubmed-meshheading:9397991-Microscopy, Immunoelectron,
pubmed-meshheading:9397991-Middle Aged,
pubmed-meshheading:9397991-Proto-Oncogene Proteins,
pubmed-meshheading:9397991-Proto-Oncogene Proteins c-myb,
pubmed-meshheading:9397991-Trans-Activators
|
| pubmed:year |
1997
|
| pubmed:articleTitle |
Hepatic expression of c-Myb in chronic human liver disease.
|
| pubmed:affiliation |
Department of Public Health, Osaka City University Medical School, Osaka, Japan.
|
| pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
|