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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
2
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pubmed:dateCreated |
1997-12-22
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pubmed:databankReference |
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pubmed:abstractText |
The unc-47 locus of Caenorhabditis elegans has been suggested to encode a synaptic vesicle GABA transporter. Here we used hydropathy plot analysis to identify a candidate vesicular GABA transporter in genomic sequences derived from a region of the physical map comprising unc-47. A mouse homologue was identified and cloned from EST database information. In situ hybridization in rat brain revealed codistribution with both GABAergic and glycinergic neuronal markers. Moreover, expression in COS-7 and PC12 cells induced an intracellular, glycine-sensitive GABA uptake activity. These observations, consistent with previous data on GABA and glycine uptake by synaptic vesicles, demonstrate that the mouse clone encodes a vesicular inhibitory amino acid transporter.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Amino Acid Transport Systems...,
http://linkedlifedata.com/resource/pubmed/chemical/Caenorhabditis elegans Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Carrier Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/GABA Plasma Membrane Transport...,
http://linkedlifedata.com/resource/pubmed/chemical/Glycine Plasma Membrane Transport...,
http://linkedlifedata.com/resource/pubmed/chemical/Membrane Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Membrane Transport Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Organic Anion Transporters,
http://linkedlifedata.com/resource/pubmed/chemical/Vesicular Inhibitory Amino Acid...,
http://linkedlifedata.com/resource/pubmed/chemical/unc-47 protein, C elegans
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pubmed:status |
MEDLINE
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pubmed:month |
Nov
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pubmed:issn |
0014-5793
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
10
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pubmed:volume |
417
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
177-83
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pubmed:dateRevised |
2006-11-20
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pubmed:meshHeading |
pubmed-meshheading:9395291-Amino Acid Sequence,
pubmed-meshheading:9395291-Amino Acid Transport Systems, Neutral,
pubmed-meshheading:9395291-Animals,
pubmed-meshheading:9395291-Base Sequence,
pubmed-meshheading:9395291-Caenorhabditis elegans,
pubmed-meshheading:9395291-Caenorhabditis elegans Proteins,
pubmed-meshheading:9395291-Carrier Proteins,
pubmed-meshheading:9395291-Cloning, Molecular,
pubmed-meshheading:9395291-Cosmids,
pubmed-meshheading:9395291-Databases, Factual,
pubmed-meshheading:9395291-GABA Plasma Membrane Transport Proteins,
pubmed-meshheading:9395291-Genes, Helminth,
pubmed-meshheading:9395291-Glycine Plasma Membrane Transport Proteins,
pubmed-meshheading:9395291-Humans,
pubmed-meshheading:9395291-In Situ Hybridization,
pubmed-meshheading:9395291-Membrane Proteins,
pubmed-meshheading:9395291-Membrane Transport Proteins,
pubmed-meshheading:9395291-Mice,
pubmed-meshheading:9395291-Molecular Sequence Data,
pubmed-meshheading:9395291-Organic Anion Transporters,
pubmed-meshheading:9395291-Rats,
pubmed-meshheading:9395291-Sequence Alignment,
pubmed-meshheading:9395291-Tissue Distribution,
pubmed-meshheading:9395291-Vesicular Inhibitory Amino Acid Transport Proteins
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pubmed:year |
1997
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pubmed:articleTitle |
Cloning of a functional vesicular GABA and glycine transporter by screening of genome databases.
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pubmed:affiliation |
CNRS ERS 575, Institut de Biologie Physico-Chimique, Paris, France.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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