Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
11
pubmed:dateCreated
1998-1-2
pubmed:abstractText
The Ly49 family of natural killer (NK) cell receptors are major histocompatibility complex (MHC) class I-specific inhibitory receptors that are distributed to overlapping NK cell subsets. Extending earlier studies of polyclonal NK cell populations, we have employed an analysis of short-term NK cell clones from Ly49A heterozygous mice, to demonstrate that the Ly49A gene is usually expressed from one or the other allele in each Ly49A+ cell. However, we also detected a small percentage of clones that expressed both Ly49A alleles. The possibility that the colonies exhibiting bi-allelic Ly49A gene expression had been inoculated with more than one cell was ruled out by parallel analysis of clones isolated from mixtures of NK cells from Ly49A homozygous mice. The frequency of bi-allelic Ly49A+ clones suggested that the two Ly49A alleles in an NK cell are chosen for expression independently. These data are consistent with the proposal that mono-allelic Ly49A gene expression may arise as a consequence of a stochastic Ly49 gene activation mechanism. Analysis of Ly49A+ clones from MHC-different mice demonstrated that class I-deficient mice harbored a greater number of bi-allelic Ly49A+ cells than did H-2d mice, which express a Ly49A ligand. Although the numbers were insufficiently large for a clear assignment, H-2b mice may harbor an intermediate number of bi-allelic Ly49A+ NK cells. The effects of MHC expression on the prevalence of bi-allelic Ly49A+ cells suggest that an MHC-dependent education process modifies the Ly49 repertoire.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Nov
pubmed:issn
0014-2980
pubmed:author
pubmed:issnType
Print
pubmed:volume
27
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
2876-84
pubmed:dateRevised
2008-11-21
pubmed:meshHeading
pubmed-meshheading:9394813-Alleles, pubmed-meshheading:9394813-Animals, pubmed-meshheading:9394813-Antigens, Ly, pubmed-meshheading:9394813-Antigens, Surface, pubmed-meshheading:9394813-Clone Cells, pubmed-meshheading:9394813-Gene Expression Regulation, pubmed-meshheading:9394813-Genes, MHC Class I, pubmed-meshheading:9394813-Killer Cells, Natural, pubmed-meshheading:9394813-Lectins, C-Type, pubmed-meshheading:9394813-Membrane Glycoproteins, pubmed-meshheading:9394813-Mice, pubmed-meshheading:9394813-Mice, Inbred BALB C, pubmed-meshheading:9394813-Mice, Inbred C57BL, pubmed-meshheading:9394813-Mice, Knockout, pubmed-meshheading:9394813-NK Cell Lectin-Like Receptor Subfamily A, pubmed-meshheading:9394813-Receptors, Immunologic, pubmed-meshheading:9394813-Receptors, NK Cell Lectin-Like, pubmed-meshheading:9394813-Transcriptional Activation
pubmed:year
1997
pubmed:articleTitle
Expression of the Ly49A gene in murine natural killer cell clones is predominantly but not exclusively mono-allelic.
pubmed:affiliation
Department of Molecular and Cell Biology, University of California at Berkeley 94720, USA.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S., Research Support, Non-U.S. Gov't