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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
12
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pubmed:dateCreated |
1998-1-2
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pubmed:abstractText |
Infection by group B streptococci (GBS) is an important cause of bacterial disease in neonates. Alpha C protein is a protective cell surface-associated protein of GBS. This protein contains a repeat region flanked by N and C termini. Variable expression of tandem repeating units of alpha C proteins had been found among clinical isolates of GBS. We examined the effect of the number of repeats on the immunogenicity of the alpha C protein and its ability to elicit protection from GBS infection in a neonatal mouse model. Mice were immunized with purified alpha C proteins of constructs containing various numbers of repeats (n = 1, 2, 9, and 16) and the N- and C-terminal regions. Both the N-terminal and the repeat regions contain protective and opsonic epitopes. Antibody responses to the alpha C protein constructs with various numbers of repeats were tested with enzyme-linked immunosorbent assay plates coated with either native, nine-repeat alpha C protein or "repeatless" N-terminal antigen. An inverse relationship was found between the number of repeats and the immunogenicity of the alpha C protein; this effect was most pronounced on titers of antibody to the N-terminal region. An inverse relationship was also observed between the number of repeats and protective efficacy, i.e., mouse dams immunized with 5 microg of one- or nine-repeat alpha C protein transferred protective immunity to 65 or 11% of their pups, respectively (P < 0.0001). Thus, the presence of multiple repeats appears to lessen the antibody response to the complete alpha C protein, and especially the antibody response to its N-terminal region, and suggests a mechanism whereby repeat elements contribute to the evasion of host immunity.
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pubmed:grant |
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/9393818-1343686,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9393818-1438195,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9393818-1500748,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9393818-1729249,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9393818-1855984,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9393818-2423947,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9393818-2460864,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9393818-276868,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9393818-3039291,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9393818-3537305,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9393818-6201506,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9393818-65433,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9393818-7822031,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9393818-7890407,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9393818-7934917,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9393818-8223489,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9393818-8326001,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9393818-8496678,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9393818-8537651,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9393818-8633028,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9393818-8751902,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9393818-8926097,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9393818-9119488
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Dec
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pubmed:issn |
0019-9567
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
65
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
5216-21
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
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pubmed:year |
1997
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pubmed:articleTitle |
Immunogenicity and protective efficacy of the alpha C protein of group B streptococci are inversely related to the number of repeats.
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pubmed:affiliation |
Channing Laboratory, Brigham and Women's Hospital, Harvard Medical School, Boston, Massachusetts 02115, USA. Claudia.Gravekamp@channing.harvard.edu
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.
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