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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
4
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pubmed:dateCreated |
1997-12-10
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pubmed:abstractText |
B7-1 (CD80) co-stimulatory molecule gene-transduced Lewis lung carcinoma (LLC) cells (LLC/B7 cells) resulted in remarkable loss of tumorigenicity in syngeneic C57BL/6 mice (87.5% rejection) compared to B7-negative, wild-type LLC (LLC/wt) cells (0% rejection). However, mice that had rejected LLC/B7 cells developed almost no systemic immunity protective against challenge with wild-type tumor cells after 4 weeks (11.8% rejection). Enhancement of MHC class I (H-2Kb) expression of LLC/B7 cells with in vitro interferon-gamma treatment did not result in enhancement of protective immunity. In vivo depletion assay revealed that abrogation of tumorigenicity in LLC/B7 depended on CD8+ T cells but not on CD4+ T cells. However, vaccination of C57BL/6 mice with irradiated LLC cells transduced with GM-CSF (LLC/GM) led to the induction of potent, specific immunity against challenge with the LLC/wt cells after 2 weeks (80.8% rejection). Next, we established a double transfectant of LLC cells expressing both B7-1 and GM-CSF (LLC/GM + B7). The tumorigenicity of these clonal cells was also remarkably suppressed (90% rejection) to the same degree as LLC/B7, whereas that of LLC/GM was not suppressed (0% rejection). Interestingly, mice that had rejected LLC/GM+B7 cells developed enhanced protective immunity against challenge with LLC/wt cells after 4 weeks (55.6% rejection) compared to the results of LLC/B7 cells (11.8%). To evaluate whether co-expression of GM-CSF and B7-1 enabled the tumor cells to activate cytotoxic T cells more efficiently than B7-1 alone, we performed an in vitro killing assay. We found that immunization with LLC/GM+B7 cells resulted in a 3-fold stronger cytotoxic response than that with LLC/B7. Our data indicate that co-transfection of the B7-1 co-stimulatory molecule and GM-CSF genes may be more effective for the induction of stronger protective immunity in this experimental system.
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pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Antigens, CD80,
http://linkedlifedata.com/resource/pubmed/chemical/Antigens, Neoplasm,
http://linkedlifedata.com/resource/pubmed/chemical/Cancer Vaccines,
http://linkedlifedata.com/resource/pubmed/chemical/DNA, Complementary,
http://linkedlifedata.com/resource/pubmed/chemical/Granulocyte-Macrophage...,
http://linkedlifedata.com/resource/pubmed/chemical/Histocompatibility Antigens Class I
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pubmed:status |
MEDLINE
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pubmed:month |
Nov
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pubmed:issn |
0020-7136
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pubmed:author | |
pubmed:issnType |
Print
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pubmed:day |
14
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pubmed:volume |
73
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
556-61
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pubmed:dateRevised |
2007-7-24
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pubmed:meshHeading |
pubmed-meshheading:9389572-Animals,
pubmed-meshheading:9389572-Antigens, CD80,
pubmed-meshheading:9389572-Antigens, Neoplasm,
pubmed-meshheading:9389572-CD4-Positive T-Lymphocytes,
pubmed-meshheading:9389572-CD8-Positive T-Lymphocytes,
pubmed-meshheading:9389572-Cancer Vaccines,
pubmed-meshheading:9389572-Carcinoma, Lewis Lung,
pubmed-meshheading:9389572-DNA, Complementary,
pubmed-meshheading:9389572-Female,
pubmed-meshheading:9389572-Graft Rejection,
pubmed-meshheading:9389572-Granulocyte-Macrophage Colony-Stimulating Factor,
pubmed-meshheading:9389572-Histocompatibility Antigens Class I,
pubmed-meshheading:9389572-Mice,
pubmed-meshheading:9389572-Mice, Inbred C57BL,
pubmed-meshheading:9389572-Neoplasm Transplantation,
pubmed-meshheading:9389572-Transfection
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pubmed:year |
1997
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pubmed:articleTitle |
GM-CSF and B7-1 (CD80) co-stimulatory signals co-operate in the induction of effective anti-tumor immunity in syngeneic mice.
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pubmed:affiliation |
Department of Hematology/Oncology, Institute of Medical Science, University of Tokyo, Japan.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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